Matches in SemOpenAlex for { <https://semopenalex.org/work/W2744078727> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W2744078727 abstract "Critical limb ischemia (CLI) is a terminal disease with high morbidity and healthcare costs due to limb loss. There are no effective medical therapies for patients with CLI to prevent amputation. Cell-based therapies are currently being investigated to address this unmet clinical need and have shown promising preliminary results. The purpose of this study was to characterize the output of a point-of-care cell separator (MarrowStim P.A.D. Kit), currently under investigation for the treatment of CLI, and compare its output with Ficoll-based separation. The outputs of the MarrowStim P.A.D. Kit and Ficoll separation were characterized using an automated hematology analyzer, colony-forming unit (CFU) assays, and tubulogenesis assays. Hematology analysis indicated that the MarrowStim P.A.D. Kit concentrated the total nucleated cells, mononuclear cells, and granulocytes compared with baseline bone marrow aspirate. Cells collected were positive for VEGFR-2, CD3, CD14, CD34, CD45, CD56, CD105, CD117, CD133, and Stro-1 antigen. CFU assays demonstrated that the MarrowStim P.A.D. Kit output a significantly greater number of mesenchymal stem cells and hematopoietic stem cells compared with cells output by Ficoll separation. There was no significant difference in the number of endothelial progenitor cells output by the two separation techniques. Isolated cells from both techniques formed interconnected nodes and microtubules in a three-dimensional cell culture assay. This information, along with data currently being collected in large-scale clinical trials, will help instruct how different cellular fractions may affect the outcomes for CLI patients." @default.
- W2744078727 created "2017-08-17" @default.
- W2744078727 creator A5025633001 @default.
- W2744078727 creator A5028666606 @default.
- W2744078727 creator A5047600403 @default.
- W2744078727 creator A5055266042 @default.
- W2744078727 creator A5073302842 @default.
- W2744078727 creator A5073352833 @default.
- W2744078727 creator A5088366640 @default.
- W2744078727 date "2015-01-01" @default.
- W2744078727 modified "2023-09-27" @default.
- W2744078727 title "Characterization of the Cellular Output of a Point-of-Care Device and the Implications for Addressing Critical Limb Ischemia" @default.
- W2744078727 hasPublicationYear "2015" @default.
- W2744078727 type Work @default.
- W2744078727 sameAs 2744078727 @default.
- W2744078727 citedByCount "0" @default.
- W2744078727 crossrefType "journal-article" @default.
- W2744078727 hasAuthorship W2744078727A5025633001 @default.
- W2744078727 hasAuthorship W2744078727A5028666606 @default.
- W2744078727 hasAuthorship W2744078727A5047600403 @default.
- W2744078727 hasAuthorship W2744078727A5055266042 @default.
- W2744078727 hasAuthorship W2744078727A5073302842 @default.
- W2744078727 hasAuthorship W2744078727A5073352833 @default.
- W2744078727 hasAuthorship W2744078727A5088366640 @default.
- W2744078727 hasConcept C10205521 @default.
- W2744078727 hasConcept C109159458 @default.
- W2744078727 hasConcept C126322002 @default.
- W2744078727 hasConcept C137061746 @default.
- W2744078727 hasConcept C142724271 @default.
- W2744078727 hasConcept C16685009 @default.
- W2744078727 hasConcept C198826908 @default.
- W2744078727 hasConcept C201750760 @default.
- W2744078727 hasConcept C202751555 @default.
- W2744078727 hasConcept C203014093 @default.
- W2744078727 hasConcept C2777466421 @default.
- W2744078727 hasConcept C2781099653 @default.
- W2744078727 hasConcept C28328180 @default.
- W2744078727 hasConcept C3018348675 @default.
- W2744078727 hasConcept C5362183 @default.
- W2744078727 hasConcept C55493867 @default.
- W2744078727 hasConcept C71924100 @default.
- W2744078727 hasConcept C86803240 @default.
- W2744078727 hasConcept C95444343 @default.
- W2744078727 hasConceptScore W2744078727C10205521 @default.
- W2744078727 hasConceptScore W2744078727C109159458 @default.
- W2744078727 hasConceptScore W2744078727C126322002 @default.
- W2744078727 hasConceptScore W2744078727C137061746 @default.
- W2744078727 hasConceptScore W2744078727C142724271 @default.
- W2744078727 hasConceptScore W2744078727C16685009 @default.
- W2744078727 hasConceptScore W2744078727C198826908 @default.
- W2744078727 hasConceptScore W2744078727C201750760 @default.
- W2744078727 hasConceptScore W2744078727C202751555 @default.
- W2744078727 hasConceptScore W2744078727C203014093 @default.
- W2744078727 hasConceptScore W2744078727C2777466421 @default.
- W2744078727 hasConceptScore W2744078727C2781099653 @default.
- W2744078727 hasConceptScore W2744078727C28328180 @default.
- W2744078727 hasConceptScore W2744078727C3018348675 @default.
- W2744078727 hasConceptScore W2744078727C5362183 @default.
- W2744078727 hasConceptScore W2744078727C55493867 @default.
- W2744078727 hasConceptScore W2744078727C71924100 @default.
- W2744078727 hasConceptScore W2744078727C86803240 @default.
- W2744078727 hasConceptScore W2744078727C95444343 @default.
- W2744078727 hasLocation W27440787271 @default.
- W2744078727 hasOpenAccess W2744078727 @default.
- W2744078727 hasPrimaryLocation W27440787271 @default.
- W2744078727 hasRelatedWork W103508879 @default.
- W2744078727 hasRelatedWork W1490988862 @default.
- W2744078727 hasRelatedWork W1976140847 @default.
- W2744078727 hasRelatedWork W2004739770 @default.
- W2744078727 hasRelatedWork W2027531937 @default.
- W2744078727 hasRelatedWork W2080052121 @default.
- W2744078727 hasRelatedWork W2097946334 @default.
- W2744078727 hasRelatedWork W2108812189 @default.
- W2744078727 hasRelatedWork W2188295643 @default.
- W2744078727 hasRelatedWork W2315738254 @default.
- W2744078727 hasRelatedWork W2318870209 @default.
- W2744078727 hasRelatedWork W2366971965 @default.
- W2744078727 hasRelatedWork W2505918952 @default.
- W2744078727 hasRelatedWork W2547444524 @default.
- W2744078727 hasRelatedWork W2563257044 @default.
- W2744078727 hasRelatedWork W2886117912 @default.
- W2744078727 hasRelatedWork W2943319557 @default.
- W2744078727 hasRelatedWork W2979504853 @default.
- W2744078727 hasRelatedWork W2980145463 @default.
- W2744078727 hasRelatedWork W3000496715 @default.
- W2744078727 isParatext "false" @default.
- W2744078727 isRetracted "false" @default.
- W2744078727 magId "2744078727" @default.
- W2744078727 workType "article" @default.