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- W2744297939 abstract "Abstract Remote ischemic preconditioning (RIPC) by repeated brief cycles of limb ischemia/reperfusion reduces myocardial ischemia/reperfusion injury. In left ventricular (LV) biopsies from patients undergoing coronary artery bypass grafting (CABG), only the activation of signal transducer and activator of transcription 5 was associated with RIPC’s cardioprotection. We have now used an unbiased, non-hypothesis-driven proteomics and phosphoproteomics approach to analyze LV biopsies from patients undergoing CABG and from pigs undergoing coronary occlusion/reperfusion without (sham) and with RIPC. False discovery rate-based statistics identified a higher prostaglandin reductase 2 expression at early reperfusion with RIPC than with sham in patients. In pigs, the phosphorylation of 116 proteins was different between baseline and early reperfusion with RIPC and/or with sham. The identified proteins were not identical for patients and pigs, but in-silico pathway analysis of proteins with ≥2-fold higher expression/phosphorylation at early reperfusion with RIPC in comparison to sham revealed a relation to mitochondria and cytoskeleton in both species. Apart from limitations of the proteomics analysis per se, the small cohorts, the sampling/sample processing and the number of uncharacterized/unverifiable porcine proteins may have contributed to this largely unsatisfactory result." @default.
- W2744297939 created "2017-08-17" @default.
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- W2744297939 date "2017-08-09" @default.
- W2744297939 modified "2023-10-14" @default.
- W2744297939 title "Proteomics/phosphoproteomics of left ventricular biopsies from patients with surgical coronary revascularization and pigs with coronary occlusion/reperfusion: remote ischemic preconditioning" @default.
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- W2744297939 doi "https://doi.org/10.1038/s41598-017-07883-5" @default.
- W2744297939 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5550488" @default.
- W2744297939 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28794502" @default.
- W2744297939 hasPublicationYear "2017" @default.
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