FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2744737291> ?p ?o ?g. }

• W2744737291 abstract "The work described herein is centered on reductive methylation, a chemical modification reaction, which allows for the introduction of methyl groups at the primary amines of the lysine side chain and at the N-terminus. The resulting methyl groups are effective probes for the study of protein structure and dynamics using nuclear magnetic resonance spectroscopy (NMR). Described herein is work conducted on the development of tools for the study of protein structure using reductive methylation, highlighting options for addressing limitations of the labeling method encountered by its practical use. Chapter 1 serves as a brief introduction to protein structure and the modification of protein structure, both biological and chemical. This chapter sets the stage for the remaining chapters by establishing the importance of structural studies and how the reductive methylation reaction has been useful to this end. Chapter 2 details work done towards the development of a method to make assignments of NMR peaks of a reductively methylated protein. The method described focuses on addressing the issue of degenerate labeling associated with the reaction, which results in similar degrees of methylation at the reaction sites. The method presented involves the introduction of solid chromatographic media into the reaction and using substoichiometric concentrations of the modifying reagents in order to introduce more variation in the labeling of the reaction sites. The overall objective of this project is to investigate if the introduction of the media and the change in reagent concentration would help to overcome the degenerate labeling and enable more definitive peak assignments. In chapter 3, methods were developed by modifying the sample environments to achieve more resolved and intense NMR peaks of reductively methylated protein. The improvement of resolution and intensity aids peak fitting for accurate peak area calculations, which are used in the determination of percent labeling at each dimethylamine peak in the NMR spectra. Chapter 4 details a study where the overall objective is to investigate the effects of reductive methylation on the determination of protein concentration by the Bradford and Bicinchoninic acid (BCA) assays. The development of a novel use of reductive methylation coupled with paramagnetic relaxation enhancement (PRE) NMR spectroscopy is detailed in Chapter 5 for the determination of the quaternary structural complex of a cysteine protease, papain, and a cysteine protease inhibitor. Chapter 6 will discuss the overall conclusions of this dissertation research and future directions of the work presented." @default.
• W2744737291 created "2017-08-17" @default.
• W2744737291 creator A5090233307 @default.
• W2744737291 date "2022-06-10" @default.
• W2744737291 modified "2023-10-15" @default.
• W2744737291 title "Method Development for the Assignment of NMR Peaks and the Determination of Quaternary Protein Structure using Reductive Methylation" @default.
• W2744737291 doi "https://doi.org/10.31390/gradschool_dissertations.2199" @default.
• W2744737291 hasPublicationYear "2022" @default.
• W2744737291 type Work @default.
• W2744737291 sameAs 2744737291 @default.
• W2744737291 citedByCount "0" @default.
• W2744737291 crossrefType "dissertation" @default.
• W2744737291 hasAuthorship W2744737291A5090233307 @default.
• W2744737291 hasBestOaLocation W27447372911 @default.
• W2744737291 hasConcept C104317684 @default.
• W2744737291 hasConcept C147597530 @default.
• W2744737291 hasConcept C178790620 @default.
• W2744737291 hasConcept C185592680 @default.
• W2744737291 hasConcept C21951064 @default.
• W2744737291 hasConcept C2776016237 @default.
• W2744737291 hasConcept C2778178838 @default.
• W2744737291 hasConcept C33288867 @default.
• W2744737291 hasConcept C40875361 @default.
• W2744737291 hasConcept C515207424 @default.
• W2744737291 hasConcept C55493867 @default.
• W2744737291 hasConcept C66974803 @default.
• W2744737291 hasConcept C91965660 @default.
• W2744737291 hasConceptScore W2744737291C104317684 @default.
• W2744737291 hasConceptScore W2744737291C147597530 @default.
• W2744737291 hasConceptScore W2744737291C178790620 @default.
• W2744737291 hasConceptScore W2744737291C185592680 @default.
• W2744737291 hasConceptScore W2744737291C21951064 @default.
• W2744737291 hasConceptScore W2744737291C2776016237 @default.
• W2744737291 hasConceptScore W2744737291C2778178838 @default.
• W2744737291 hasConceptScore W2744737291C33288867 @default.
• W2744737291 hasConceptScore W2744737291C40875361 @default.
• W2744737291 hasConceptScore W2744737291C515207424 @default.
• W2744737291 hasConceptScore W2744737291C55493867 @default.
• W2744737291 hasConceptScore W2744737291C66974803 @default.
• W2744737291 hasConceptScore W2744737291C91965660 @default.
• W2744737291 hasLocation W27447372911 @default.
• W2744737291 hasOpenAccess W2744737291 @default.
• W2744737291 hasPrimaryLocation W27447372911 @default.
• W2744737291 hasRelatedWork W1099221832 @default.
• W2744737291 hasRelatedWork W2017159870 @default.
• W2744737291 hasRelatedWork W2019155595 @default.
• W2744737291 hasRelatedWork W2023630079 @default.
• W2744737291 hasRelatedWork W2032817846 @default.
• W2744737291 hasRelatedWork W2060882402 @default.
• W2744737291 hasRelatedWork W2140354277 @default.
• W2744737291 hasRelatedWork W2547642576 @default.
• W2744737291 hasRelatedWork W2949802403 @default.
• W2744737291 hasRelatedWork W953729605 @default.
• W2744737291 isParatext "false" @default.
• W2744737291 isRetracted "false" @default.
• W2744737291 magId "2744737291" @default.
• W2744737291 workType "dissertation" @default.