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• W2745296631 abstract "2‐Phenylethanol (2PE) is a key molecule used in the fragrance and food industries, as well as a potential biofuel. In contrast to its extraction from plant biomass and/or more common chemical synthesis, microbial 2PE production has been demonstrated via both native and heterologous expression of the yeast Ehrlich pathway. Here, a novel alternative to this established pathway is systematically engineered in Escherichia coli and evaluated as a more robust and efficient route. This novel pathway is constructed via the modular extension of a previously engineered styrene biosynthesis pathway, proceeding from endogenous l ‐phenylalanine in five steps and involving four heterologous enzymes. This “styrene‐derived” pathway boasts nearly a 10‐fold greater thermodynamic driving force than the Ehrlich pathway, and enables reduced accumulation of acetate byproduct. When directly compared using a host strain engineered for l ‐phenylalanine over‐production, preservation of phosphoenolpyruvate, and reduced formation of byproduct 2‐phenylacetic acid, final 2PE titers via the styrene‐derived and Ehrlich pathways reached 1817 and 1164 mg L −1 , respectively, at yields of 60.6 and 38.8 mg g −1 . Following optimization of induction timing and initial glucose loading, 2PE titers by the styrene‐derived pathway approached 2 g L −1 – nearly a two‐fold twofold increase over prior reports for 2PE production by E. coli employing the Ehrlich pathway." @default.
• W2745296631 created "2017-08-17" @default.
• W2745296631 creator A5000323280 @default.
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• W2745296631 date "2017-09-04" @default.
• W2745296631 modified "2023-10-05" @default.
• W2745296631 title "Expanding Upon Styrene Biosynthesis to Engineer a Novel Route to 2‐Phenylethanol" @default.
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• W2745296631 doi "https://doi.org/10.1002/biot.201700310" @default.
• W2745296631 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28799719" @default.
• W2745296631 hasPublicationYear "2017" @default.
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