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- W2745724300 abstract "Currently, isothermal methods of nucleic acid amplification have been well established; in particular, rolling circle amplification is of great interest. In this approach, circular ssDNA molecules have been used as a target that can be obtained by the intramolecular template-dependent ligation of an oligonucleotide C-probe. Here, a new method of synthesizing small circular DNA molecules via the cyclization of ssDNA based on T4 RNA ligase has been proposed. Circular ssDNA is further used as the template for the rolling circle amplification. The maximum yield of the cyclization products was observed in the presence of 5-10% polyethylene glycol 4000, and the optimum DNA length for the cyclization constituted 50 nucleotides. This highly sensitive method was shown to detect less than 10^(2) circular DNA molecules. The method reliability was proved based on artificially destroyed dsDNA, which suggests its implementation for analyzing any significantly fragmented dsDNA." @default.
- W2745724300 created "2017-08-31" @default.
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- W2745724300 date "2017-01-01" @default.
- W2745724300 modified "2023-09-25" @default.
- W2745724300 title "ПОЛУЧЕНИЕ ДНК-МАТРИЦ С ПОМОЩЬЮ Т4 РНК-ЛИГАЗЫ ДЛЯ АМПЛИФИКАЦИИ КАТЯЩИМСЯ КОЛЬЦОМ, Молекулярная биология" @default.
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- W2745724300 doi "https://doi.org/10.7868/s0026898417040164" @default.
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