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- W2745791037 abstract "A loop-mediated isothermal amplification (LAMP) assay was used for rapid canine parvovirus (CPV) diagnosis. To reduce the time required and increase the sensitivity of the assay, an immunocapture (IC) technique was developed in this study to exclude the DNA extraction step in molecular diagnostic procedures for CPV. A polyclonal rabbit anti-CPV serum was produced against VP2-EpC that was cloned via DNA recombination. The polyclonal anti-VP2-EpC serum was used for virus capture to prepare microtubes. IC-LAMP was performed to amplify a specific CPV target gene sequence from the CPV viral particles that were captured on the microtubes, and the amplicons were analyzed using agarose electrophoresis or enzyme-linked immunosorbent assay (IC-LAMP-ELISA) and lateral-flow dipstick (IC-LAMP-LFD). The detection sensitivities of IC-LAMP, IC-LAMP-ELISA, and IC-LAMP-LFD were 10−1, 10−1, and 10−1 TCID50/mL, respectively. Using the IC-LAMP-ELISA and IC-LAMP-LFD assays, the complete CPV diagnostic process can be achieved within 1.5 h. Both of the developed IC-LAMP-based assays are simple, direct visual and efficient techniques that are applicable to the detection of CPV." @default.
- W2745791037 created "2017-08-31" @default.
- W2745791037 creator A5060837534 @default.
- W2745791037 creator A5066777156 @default.
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- W2745791037 date "2017-11-01" @default.
- W2745791037 modified "2023-10-14" @default.
- W2745791037 title "Immunocapture loop-mediated isothermal amplification assays for the detection of canine parvovirus" @default.
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- W2745791037 doi "https://doi.org/10.1016/j.jviromet.2017.08.009" @default.
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