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- W2745907774 abstract "In skeletal muscle, DNA methylation contributes to the suppression of gene expression in several biological processes and diseases. A protocol for the detection of methylated cytosine was thus established based on methylation-sensitive enzymes, immunoprecipitation, and bisulfite conversion. DNA methylation analysis, with bisulfite conversion and sequencing, enables the quantification of methylation at each single base position. Here, we describe a basic method of bisulfite sequencing that can be used to analyze local DNA methylation status to confirm genome-wide DNA methylation analysis or correlation of gene expression regulatory mechanisms." @default.
- W2745907774 created "2017-08-31" @default.
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- W2745907774 date "2017-01-01" @default.
- W2745907774 modified "2023-09-24" @default.
- W2745907774 title "Bisulfite Sequencing for DNA Methylation Analysis of Primary Muscle Stem Cells" @default.
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- W2745907774 doi "https://doi.org/10.1007/978-1-4939-7283-8_1" @default.
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