FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2746281036> ?p ?o ?g. }

• W2746281036 endingPage "637" @default.
• W2746281036 startingPage "625" @default.
• W2746281036 abstract "miRNAs represent an emerging class of promising biomarkers for cancer diagnostics. To perform reliable miRNA expression analysis using quantitative PCR, adequate data normalization is essential to remove nonbiological, technical variations. Ideal reference genes should be biologically stable and reduce technical variability of miRNA expression analysis. Herein is a new strategy for the identification and evaluation of reference genes that can be applied for miRNA-based diagnostic tests without entailing excessive additional experiments. We analyzed the expression of 11 carefully selected candidate reference genes in different types of cervical specimens [ie, tissues, scrapes, and self-collected cervicovaginal specimens (self-samples)]. To identify the biologically most stable reference genes, three commonly used algorithms (GeNorm, NormFinder, and BestKeeper) were combined. Signal-to-noise ratios and P values between control and disease groups were calculated to validate the reduction in technical variability on expression analysis of two marker miRNAs. miR-423 was identified as a suitable reference gene for all sample types, to be used in combination with RNU24 in cervical tissues, RNU43 in scrapes, and miR-30b in self-samples. These findings demonstrate that the choice of reference genes may differ between different types of specimens, even when originating from the same anatomical source. More important, it is shown that adequate normalization increases the signal-to-noise ratio, which is not observed when normalizing to commonly used reference genes." @default.
• W2746281036 created "2017-08-31" @default.
• W2746281036 creator A5036462844 @default.
• W2746281036 creator A5048187760 @default.
• W2746281036 creator A5057632118 @default.
• W2746281036 creator A5061099291 @default.
• W2746281036 creator A5082492592 @default.
• W2746281036 date "2017-09-01" @default.
• W2746281036 modified "2023-09-25" @default.
• W2746281036 title "A Strategy to Find Suitable Reference Genes for miRNA Quantitative PCR Analysis and Its Application to Cervical Specimens" @default.
• W2746281036 cites W1577577364 @default.
• W2746281036 cites W1853245536 @default.
• W2746281036 cites W1969192247 @default.
• W2746281036 cites W1974254202 @default.
• W2746281036 cites W1998431482 @default.
• W2746281036 cites W1999638394 @default.
• W2746281036 cites W2006369674 @default.
• W2746281036 cites W2017777108 @default.
• W2746281036 cites W2019766856 @default.
• W2746281036 cites W2025194826 @default.
• W2746281036 cites W2025492442 @default.
• W2746281036 cites W2032411253 @default.
• W2746281036 cites W2041983200 @default.
• W2746281036 cites W2049435756 @default.
• W2746281036 cites W2061975704 @default.
• W2746281036 cites W2071593083 @default.
• W2746281036 cites W2078757557 @default.
• W2746281036 cites W2081339873 @default.
• W2746281036 cites W2094647473 @default.
• W2746281036 cites W2099114361 @default.
• W2746281036 cites W2099451994 @default.
• W2746281036 cites W2099824652 @default.
• W2746281036 cites W2100651747 @default.
• W2746281036 cites W2100869063 @default.
• W2746281036 cites W2104956231 @default.
• W2746281036 cites W2105423509 @default.
• W2746281036 cites W2107018762 @default.
• W2746281036 cites W2107277218 @default.
• W2746281036 cites W2107398329 @default.
• W2746281036 cites W2114911247 @default.
• W2746281036 cites W2115424432 @default.
• W2746281036 cites W2120865735 @default.
• W2746281036 cites W2121732950 @default.
• W2746281036 cites W2127535576 @default.
• W2746281036 cites W2141549067 @default.
• W2746281036 cites W2144175542 @default.
• W2746281036 cites W2147241696 @default.
• W2746281036 cites W2148630375 @default.
• W2746281036 cites W2149106795 @default.
• W2746281036 cites W2149966907 @default.
• W2746281036 cites W2151501050 @default.
• W2746281036 cites W2151868278 @default.
• W2746281036 cites W2156683104 @default.
• W2746281036 cites W2157594971 @default.
• W2746281036 cites W2157981625 @default.
• W2746281036 cites W2160918828 @default.
• W2746281036 cites W2162359611 @default.
• W2746281036 cites W2166842249 @default.
• W2746281036 cites W2168420558 @default.
• W2746281036 cites W2267454507 @default.
• W2746281036 cites W2308074142 @default.
• W2746281036 cites W4214489112 @default.
• W2746281036 doi "https://doi.org/10.1016/j.jmoldx.2017.04.010" @default.
• W2746281036 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28826607" @default.
• W2746281036 hasPublicationYear "2017" @default.
• W2746281036 type Work @default.
• W2746281036 sameAs 2746281036 @default.
• W2746281036 citedByCount "19" @default.
• W2746281036 countsByYear W27462810362018 @default.
• W2746281036 countsByYear W27462810362019 @default.
• W2746281036 countsByYear W27462810362020 @default.
• W2746281036 countsByYear W27462810362021 @default.
• W2746281036 countsByYear W27462810362022 @default.
• W2746281036 countsByYear W27462810362023 @default.
• W2746281036 crossrefType "journal-article" @default.
• W2746281036 hasAuthorship W2746281036A5036462844 @default.
• W2746281036 hasAuthorship W2746281036A5048187760 @default.
• W2746281036 hasAuthorship W2746281036A5057632118 @default.
• W2746281036 hasAuthorship W2746281036A5061099291 @default.
• W2746281036 hasAuthorship W2746281036A5082492592 @default.
• W2746281036 hasBestOaLocation W27462810361 @default.
• W2746281036 hasConcept C104317684 @default.
• W2746281036 hasConcept C121608353 @default.
• W2746281036 hasConcept C136886441 @default.
• W2746281036 hasConcept C144024400 @default.
• W2746281036 hasConcept C145059251 @default.
• W2746281036 hasConcept C150194340 @default.
• W2746281036 hasConcept C153180895 @default.
• W2746281036 hasConcept C154945302 @default.
• W2746281036 hasConcept C162984825 @default.
• W2746281036 hasConcept C18431079 @default.
• W2746281036 hasConcept C19165224 @default.
• W2746281036 hasConcept C2778220009 @default.
• W2746281036 hasConcept C2779148672 @default.
• W2746281036 hasConcept C41008148 @default.
• W2746281036 hasConcept C48023723 @default.
• W2746281036 hasConcept C54355233 @default.
• W2746281036 hasConcept C60644358 @default.

• next