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- W2746446880 abstract "To investigate the inhibition of cell proliferation by essential oil of Chenopodium ambrosioides on the human liver cancer SMMC-7721 cells and human liver LO2 cells,and to study the mechanism of anti-tumor in vitro.The inhibition of cell proliferation by essential oil of Chenopodium ambrosioides was determined by MTT assay; the distribution of cell cycle was analyzed by flow cytometry( FCM) with PI staining; cell morphology and apoptosis effect of SMMC-7721 cells were observed by microscope; the apoptotic rate was quantified by FCM with Annexin V / PI double staining.Essential oil of Chenopodium ambrosioides could significantly inhibit the cell proliferation in a concentration-time-dependent manner( P < 0. 05),and the IC50 values on SMMC-7721 cells were lower than human liver LO2 cells at 24,48 and 72 h,respectively( P < 0. 05); cell cycle of SMMC-7721 cells was arrested in G0/G1phase; morphological observation revealed that the cells were wrinkled and the cellular cohesiveness of cells was reduced; nuclear was condensed and in orange colour,of which were the late apoptotic features; and the apoptotic rate increased in a concentration-dependent manner( P < 0. 05),non-viable apoptotic rate was obviously decreased with caspase inhibitor in 100 μg / m L essential oil of Chenopodium ambrosioides( P < 0. 01).Essential oil of Chenopodium ambrosioides can inhibit SMMC-7721 cell proliferation, which may be related to inducing cell cycle arrest and caspase-dependent apoptosis." @default.
- W2746446880 created "2017-08-31" @default.
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- W2746446880 date "2016-05-01" @default.
- W2746446880 modified "2023-09-22" @default.
- W2746446880 title "[Essential Oil of Chenopodium ambrosioides Induced Caspase-Dependent Apoptosis in SMMC-7721 Cells]." @default.
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