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- W2747501091 abstract "Abstract Carotenoid deposition in gac fruit aril, carrot root, and tomato fruit was investigated in situ using a combination of light microscopy, UV/Vis transmission spectroscopy, and diffuse reflectance spectroscopy. Reflectance data was transformed to absorbance data using the Kubelka-Munk function. As shown by light microscopy, carotenoids of gac fruit aril were deposited in round-shaped chromoplasts, whereas tomato and carrot were characterized by needle-shaped, crystalloid chromoplasts. Crystalline carotenoid deposition in tomato and carrot could be confirmed via polarized light microscopy. By identical means, crystalline deposition of lycopene and β-carotene in gac fruit aril was revealed, most likely in form of microcrystalline aggregates. This hypothesis was supported by a simple solubility estimation based on carotenoid and lipid contents in gac fruit aril. By comparison of UV/Vis absorption spectra recorded from aggregated genuine carotenoid extracts, authentic standards, and transformed reflectance spectra, an H-aggregated form of lycopene in tomato, J-aggregated forms of carotenoids in carrot, and a combination of H-aggregated lycopene, J-aggregated β-carotene, and possibly lipid-dissolved carotenoids in gac fruit aril was confirmed. The present approach was used to gain insights into the aggregate forms of carotenoids in planta , representing a low-cost, non-destructive in situ -characterization method." @default.
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- W2747501091 date "2018-01-01" @default.
- W2747501091 modified "2023-09-24" @default.
- W2747501091 title "Non-destructive approach for the characterization of the in situ carotenoid deposition in gac fruit aril" @default.
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- W2747501091 doi "https://doi.org/10.1016/j.jfca.2017.08.006" @default.
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