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- W2747692016 abstract "ObjectiveTo observe the effect of bone morphogenetic proteins (BMP)-7 on the metabo-lism of rat chondrocytes under inflammatory stimulation with interleukin (IL)-1β in vitro.MethodsThe effect of different concentrations of BMP-7 (50 ng/ml, 100 ng/ml, 200 ng/ml) with or without different concentrations of glucosamine (5%, 10%, 20%) on IL-1β-inducing change of the chondrocytes cell proliferationwas detected by CCK-8 colorimetric assay. After treatment with 100 ng/ml BMP-7, 10% glucosamine and 50 ng/ml BMP-7 plus 10% glucosamine respectively, the impact of type Ⅱ collagen, aggrecan and matrix metalloproteinases (MMP)-13 expression changes induced by IL-1β was determined by real-time polymerase chain reaction (PCR) and immunofluorescence from the level of mRNA and protein. The variance of the groups was compared with LSD method, and the variance was not homogeneous by Tamhane T2 method.ResultsAfter stimulated with IL-1β for 24 hours, the proliferation of chondrocytes was inhibited significantly (993±53, P<0.05), and the mRNA expression of type Ⅱ collagen and aggrecan were decreased (0.42±0.04 and 0.82±0.06, P<0.05) while MMP-13 was increased (1.01±0.09, P<0.05), accompanied by the declining protein expression of type Ⅱ collagen and aggrecan (1.26±0.11 and 1.38±0.11, P<0.05). BMP-7 improved the IL-β-inducing inhibitory of chondrocytes proliferation(4 052±140, P<0.05) and reversed type Ⅱ collagen, aggrecan expression changed from the level of mRNA(1.46±0.19 and 1.60±0.07, P<0.05) and type Ⅱ collagen, aggrecan, MMP-13 expression changed from the level of protein(0.94±0.23, 1.58±0.11 and 0.89±0.07, both of them P<0.05).ConclusionBMP-7 is able to improve the effect of IL-1β on the metabolism of rat chondrocytes in vitro.Key words: Chondrocytes; Osteoarthritis; Bone morphogenetic proteins-7" @default.
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- W2747692016 date "2016-11-15" @default.
- W2747692016 modified "2023-09-24" @default.
- W2747692016 title "Effects of bone morphogenetic proteins-7 on the metabolism of rat chondrocytes in vitro" @default.
- W2747692016 doi "https://doi.org/10.3760/cma.j.issn.1007-7480.2016.11.011" @default.
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