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- W2748156711 abstract "In this study, a pH shock strategy was employed to enhance ε-poly-l-lysine (ε-PL) production from glucose. In the conventional fermentation, in the early stage, only 13% of total ε-PL production is achieved in 25% of the entire fermentation period, which severely affected ε-PL productivity. To improve the efficiency of ε-PL production during fermentation, a novel two-stage fermentation, namely culture and fermentation stages, was proposed on the basis of the analysis of conventional pH shock fermentation. After optimization of parameters such as inoculum growth conditions, initial fermentation pH, and inoculum volume, the ε-PL production and productivity achieved using the novel fermentation process in a 5-L fermenter reached 32.22 g/L and 5.86 g/L/day, which were 32.3% and 36.6% higher, respectively, when compared with those obtained in conventional fermentation. Furthermore, evaluation of acid tolerance of mycelia collected from the pH shock fermentation showed that pH shock enhanced ε-PL production, which might be related to the acid tolerance of Streptomyces albulus and pH stress (pH 3.0). The results obtained could be useful for large-scale ε-PL production and to provide new information on ε-PL biosynthesis mechanism." @default.
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- W2748156711 date "2017-12-01" @default.
- W2748156711 modified "2023-10-15" @default.
- W2748156711 title "Efficient production of ε-poly-l-lysine from glucose by two-stage fermentation using pH shock strategy" @default.
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- W2748156711 doi "https://doi.org/10.1016/j.procbio.2017.08.008" @default.
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