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• W2748804917 abstract "Lignocellulolytic microorganisms were isolated from cow manure, the soil from naturally grown bamboo, the piles of rubber bark and the composting piles. The samples were collected at 9 different locations which were 5 cm deep from the surface. The basal medium (BM) containing CMC (carboxymethyl cellulose) or xylan as a carbon source and the lignin modifying basal medium (LBM) with ABTS {2,2¢-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid} or DMP (2,6-dimethoxyphenol) as an inducer were used for the primary screening of microorganisms producing cellulase, xylanase, laccase and manganese peroxidase, respectively. Forty-five bacterial isolates (B), twenty-five isolates of actinomyces (A) and fifteen fungal isolates (F) were obtained. After that twelve isolates were selected to evaluate enzyme production in LBM containing decomposed rubber bark as a carbon source at 30 °C and at 45 °C under aerobic conditions (150 rpm shaking). The isolates F6, A2 and B15 displayed the highest enzyme activities with a CMCase activity of 0.61, 0.33 and 0.21 U/mL, respectively. Moreover, they also produced xylanase with an activity of 0.61, 0.36 and 0.32 U/mL, respectively. However, only the isolate F6 showed laccase activity and manganese peroxidase activity of 0.81 and 0.56 U/mL, respectively. The isolates B15 and A2 were identified by 16S rDNA with 99 % similarity to Bacillus subtilis and Streptomyces thermovulgaris , respectively. Aside from that, isolate F6 was identified by 18S rDNA with 100 % similarity to Trichoderma asperellum . When these microorganisms were grown in the LBM broth with 10 % decomposed rubber bark they produced the highest lignocellulolytic enzymes in the stationary phase of growth." @default.
• W2748804917 created "2017-08-31" @default.
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• W2748804917 date "2017-06-15" @default.
• W2748804917 modified "2023-09-26" @default.
• W2748804917 title "Isolation and Screening of Potential Lignocellulolytic Microorganisms from Rubber Bark and Other Agricultural Residues" @default.
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