Matches in SemOpenAlex for { <https://semopenalex.org/work/W2749000131> ?p ?o ?g. }
- W2749000131 endingPage "7735" @default.
- W2749000131 startingPage "7726" @default.
- W2749000131 abstract "Trehalose has important applications in the food industry and pharmaceutical manufacturing. The thermostable enzyme trehalose synthase from Thermobaculum terrenum (TtTS) catalyzes the reversible interconversion of maltose and trehalose. Here, we investigated the structural characteristics of TtTS in complex with the inhibitor TriS. TtTS exhibits the typical three domain glycoside hydrolase family 13 structure. The catalytic cleft consists of Asp202-Glu244-Asp310 and various conserved substrate-binding residues. However, among trehalose synthases, TtTS demonstrates obvious thermal stability. TtTS has more polar (charged) amino acids distributed on its protein structure surface and more aromatic amino acids buried within than other mesophilic trehalose synthases. Furthermore, TtTS structural analysis revealed four potential metal ion-binding sites rather than the two in a homologous structure. These factors may render TtTS relatively more thermostable among mesophilic trehalose synthases. The detailed thermophilic enzyme structure provided herein may provide guidance for further protein engineering in the design of stabilized enzymes." @default.
- W2749000131 created "2017-08-31" @default.
- W2749000131 creator A5007588772 @default.
- W2749000131 creator A5034562348 @default.
- W2749000131 creator A5045228160 @default.
- W2749000131 creator A5052902867 @default.
- W2749000131 creator A5085856281 @default.
- W2749000131 date "2017-08-23" @default.
- W2749000131 modified "2023-10-11" @default.
- W2749000131 title "Structural Characteristics and Function of a New Kind of Thermostable Trehalose Synthase from <i>Thermobaculum terrenum</i>" @default.
- W2749000131 cites W1509113763 @default.
- W2749000131 cites W1517556202 @default.
- W2749000131 cites W1539796472 @default.
- W2749000131 cites W1562537255 @default.
- W2749000131 cites W1816602264 @default.
- W2749000131 cites W1894646060 @default.
- W2749000131 cites W1970967867 @default.
- W2749000131 cites W1971675810 @default.
- W2749000131 cites W1976976759 @default.
- W2749000131 cites W1983625008 @default.
- W2749000131 cites W1985579908 @default.
- W2749000131 cites W1986191025 @default.
- W2749000131 cites W1987489304 @default.
- W2749000131 cites W1989271561 @default.
- W2749000131 cites W1996661126 @default.
- W2749000131 cites W2007522065 @default.
- W2749000131 cites W2017439787 @default.
- W2749000131 cites W2026229448 @default.
- W2749000131 cites W2029361381 @default.
- W2749000131 cites W2032982721 @default.
- W2749000131 cites W2037760587 @default.
- W2749000131 cites W2041555931 @default.
- W2749000131 cites W2048104427 @default.
- W2749000131 cites W2053011453 @default.
- W2749000131 cites W2060818115 @default.
- W2749000131 cites W2066564362 @default.
- W2749000131 cites W2074093995 @default.
- W2749000131 cites W2079996771 @default.
- W2749000131 cites W2082042773 @default.
- W2749000131 cites W2086779432 @default.
- W2749000131 cites W2099057076 @default.
- W2749000131 cites W2106413031 @default.
- W2749000131 cites W2106935782 @default.
- W2749000131 cites W2108605550 @default.
- W2749000131 cites W2108921801 @default.
- W2749000131 cites W2109184569 @default.
- W2749000131 cites W2110869427 @default.
- W2749000131 cites W2113034597 @default.
- W2749000131 cites W2116466817 @default.
- W2749000131 cites W2120230374 @default.
- W2749000131 cites W2130209509 @default.
- W2749000131 cites W2137639755 @default.
- W2749000131 cites W2137825366 @default.
- W2749000131 cites W2141003340 @default.
- W2749000131 cites W2143596010 @default.
- W2749000131 cites W2143862901 @default.
- W2749000131 cites W2144081223 @default.
- W2749000131 cites W2146983134 @default.
- W2749000131 cites W2154332045 @default.
- W2749000131 cites W2156350829 @default.
- W2749000131 cites W2165940462 @default.
- W2749000131 doi "https://doi.org/10.1021/acs.jafc.7b02732" @default.
- W2749000131 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28809106" @default.
- W2749000131 hasPublicationYear "2017" @default.
- W2749000131 type Work @default.
- W2749000131 sameAs 2749000131 @default.
- W2749000131 citedByCount "22" @default.
- W2749000131 countsByYear W27490001312018 @default.
- W2749000131 countsByYear W27490001312019 @default.
- W2749000131 countsByYear W27490001312020 @default.
- W2749000131 countsByYear W27490001312021 @default.
- W2749000131 countsByYear W27490001312022 @default.
- W2749000131 countsByYear W27490001312023 @default.
- W2749000131 crossrefType "journal-article" @default.
- W2749000131 hasAuthorship W2749000131A5007588772 @default.
- W2749000131 hasAuthorship W2749000131A5034562348 @default.
- W2749000131 hasAuthorship W2749000131A5045228160 @default.
- W2749000131 hasAuthorship W2749000131A5052902867 @default.
- W2749000131 hasAuthorship W2749000131A5085856281 @default.
- W2749000131 hasConcept C147816474 @default.
- W2749000131 hasConcept C181199279 @default.
- W2749000131 hasConcept C185592680 @default.
- W2749000131 hasConcept C196032511 @default.
- W2749000131 hasConcept C2778197599 @default.
- W2749000131 hasConcept C2778452849 @default.
- W2749000131 hasConcept C2781216036 @default.
- W2749000131 hasConcept C27911776 @default.
- W2749000131 hasConcept C515207424 @default.
- W2749000131 hasConcept C55493867 @default.
- W2749000131 hasConceptScore W2749000131C147816474 @default.
- W2749000131 hasConceptScore W2749000131C181199279 @default.
- W2749000131 hasConceptScore W2749000131C185592680 @default.
- W2749000131 hasConceptScore W2749000131C196032511 @default.
- W2749000131 hasConceptScore W2749000131C2778197599 @default.
- W2749000131 hasConceptScore W2749000131C2778452849 @default.
- W2749000131 hasConceptScore W2749000131C2781216036 @default.
- W2749000131 hasConceptScore W2749000131C27911776 @default.
- W2749000131 hasConceptScore W2749000131C515207424 @default.