FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2749156769> ?p ?o ?g. }

• W2749156769 abstract "Abstract To investigate the ability of SAHA-induced TRAIL DR5-CTSB crosstalk to initiate the breast cancer autophagy, RTCA assay was performed to assess the effect of SAHA on breast cancer cells, and western blot and ELISA were used to verify the inductive effects on expression of CTSB. Breast cancer cells were transfected with TRAIL DR5 siRNA to block the function of TRAIL DR5. Cell viability and apoptosis of breast cancer cells were analyzed using a muse cell analyzer. The distribution of LC3-II in TRAIL DR5-silenced breast cancer cells treated with SAHA was observed by immunofluorescence microscopy, the mRNA levels of autophagy-related genes were detected by RNA microarray, and the activity of autophagy-related signaling pathways was screened by MAPK antibody array. Results indicated that SAHA did indeed repress the growth of breast cancer cell lines with inducing CTSB expression. Western blot and ELISA results indicated that TRAIL DR5 was involved in the expression of CTSB in SAHA-induced breast cancer cells. Cell viability and apoptosis assays showed that the inactivation of TRAIL DR5 can significantly inhibit the effects of SAHA. An immunofluorescence assay indicated that, with SAHA treatment, MDA-MB-231 and MCF-7 cells underwent apparent morphological changes. While SAHA was added in the TRAIL-DR5 blocked cells, the distribution of LC3-II signal was dispersed, the intensity of fluorescence signal was weaker than that of SAHA alone. RNA array indicated that SAHA significantly increased mRNA expression of autophagy marker LC3A/B whereas the change was significantly reversed in TRAIL DR5-silenced cells. The results of MAPK antibody array showed that SAHA and TRAIL DR5 could affect the activity of AKT1, AKT2, and TOR protein in breast cancer cells. These results provide more evidence that SAHA may stimulate TRAIL DR5-CTSB crosstalk, influence the activity of downstream TOR signalling pathway mainly through the AKTs pathway, and initiate the autophagy of breast cancer cells." @default.
• W2749156769 created "2017-08-31" @default.
• W2749156769 creator A5002864551 @default.
• W2749156769 creator A5011480176 @default.
• W2749156769 creator A5012677271 @default.
• W2749156769 creator A5020631342 @default.
• W2749156769 creator A5039125561 @default.
• W2749156769 creator A5089909886 @default.
• W2749156769 date "2017-08-21" @default.
• W2749156769 modified "2023-10-12" @default.
• W2749156769 title "TRAIL DR5-CTSB crosstalk participates in breast cancer autophagy initiated by SAHA" @default.
• W2749156769 cites W1563500585 @default.
• W2749156769 cites W1621223622 @default.
• W2749156769 cites W1769546429 @default.
• W2749156769 cites W1965750396 @default.
• W2749156769 cites W1966980951 @default.
• W2749156769 cites W1981035185 @default.
• W2749156769 cites W1991105254 @default.
• W2749156769 cites W1993643473 @default.
• W2749156769 cites W1996745688 @default.
• W2749156769 cites W2004861601 @default.
• W2749156769 cites W2006271108 @default.
• W2749156769 cites W2009085926 @default.
• W2749156769 cites W2012758939 @default.
• W2749156769 cites W2016394200 @default.
• W2749156769 cites W2034132952 @default.
• W2749156769 cites W2039419943 @default.
• W2749156769 cites W2046176913 @default.
• W2749156769 cites W2057813271 @default.
• W2749156769 cites W2066141091 @default.
• W2749156769 cites W2069875612 @default.
• W2749156769 cites W2069938223 @default.
• W2749156769 cites W2073059521 @default.
• W2749156769 cites W2076263734 @default.
• W2749156769 cites W2086384126 @default.
• W2749156769 cites W2100605768 @default.
• W2749156769 cites W2104902173 @default.
• W2749156769 cites W2128779315 @default.
• W2749156769 cites W2138509357 @default.
• W2749156769 cites W2139354073 @default.
• W2749156769 cites W2146415848 @default.
• W2749156769 cites W2323165065 @default.
• W2749156769 cites W2347004732 @default.
• W2749156769 cites W2426034980 @default.
• W2749156769 cites W2500935108 @default.
• W2749156769 cites W2518558834 @default.
• W2749156769 cites W2527423483 @default.
• W2749156769 cites W2530601644 @default.
• W2749156769 cites W2543900640 @default.
• W2749156769 cites W2545511601 @default.
• W2749156769 cites W2558764453 @default.
• W2749156769 cites W2570618306 @default.
• W2749156769 cites W2587560288 @default.
• W2749156769 cites W2620241325 @default.
• W2749156769 cites W4302086458 @default.
• W2749156769 doi "https://doi.org/10.1038/cddiscovery.2017.52" @default.
• W2749156769 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5629629" @default.
• W2749156769 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29018571" @default.
• W2749156769 hasPublicationYear "2017" @default.
• W2749156769 type Work @default.
• W2749156769 sameAs 2749156769 @default.
• W2749156769 citedByCount "6" @default.
• W2749156769 countsByYear W27491567692019 @default.
• W2749156769 countsByYear W27491567692020 @default.
• W2749156769 countsByYear W27491567692021 @default.
• W2749156769 countsByYear W27491567692022 @default.
• W2749156769 crossrefType "journal-article" @default.
• W2749156769 hasAuthorship W2749156769A5002864551 @default.
• W2749156769 hasAuthorship W2749156769A5011480176 @default.
• W2749156769 hasAuthorship W2749156769A5012677271 @default.
• W2749156769 hasAuthorship W2749156769A5020631342 @default.
• W2749156769 hasAuthorship W2749156769A5039125561 @default.
• W2749156769 hasAuthorship W2749156769A5089909886 @default.
• W2749156769 hasBestOaLocation W27491567691 @default.
• W2749156769 hasConcept C104317684 @default.
• W2749156769 hasConcept C121608353 @default.
• W2749156769 hasConcept C126322002 @default.
• W2749156769 hasConcept C1491633281 @default.
• W2749156769 hasConcept C153911025 @default.
• W2749156769 hasConcept C159654299 @default.
• W2749156769 hasConcept C185592680 @default.
• W2749156769 hasConcept C190283241 @default.
• W2749156769 hasConcept C203014093 @default.
• W2749156769 hasConcept C203522944 @default.
• W2749156769 hasConcept C2776415932 @default.
• W2749156769 hasConcept C2778236600 @default.
• W2749156769 hasConcept C502942594 @default.
• W2749156769 hasConcept C530470458 @default.
• W2749156769 hasConcept C53227056 @default.
• W2749156769 hasConcept C54009773 @default.
• W2749156769 hasConcept C54355233 @default.
• W2749156769 hasConcept C55493867 @default.
• W2749156769 hasConcept C62112901 @default.
• W2749156769 hasConcept C71924100 @default.
• W2749156769 hasConcept C81885089 @default.
• W2749156769 hasConcept C86803240 @default.
• W2749156769 hasConcept C96232424 @default.
• W2749156769 hasConceptScore W2749156769C104317684 @default.
• W2749156769 hasConceptScore W2749156769C121608353 @default.
• W2749156769 hasConceptScore W2749156769C126322002 @default.

• next