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- W2750082928 abstract "The plant-specific PALE CRESS (PAC) protein has previously been shown to be essential for photoautotrophic growth. Here we further investigated the molecular function of the PAC protein. PAC localizes to plastid nucleoids and forms large proteinaceous and RNA-containing megadalton complexes. It co-immunoprecipitates with a specific subset of chloroplast RNAs including psbK-psbI, ndhF, ndhD, and 23S ribosomal RNA (rRNA), as demonstrated by RNA immunoprecipitation in combination with high throughput RNA sequencing (RIP-seq) analyses. Furthermore, it co-migrates with premature 50S ribosomal particles and specifically binds to 23S rRNA in vitro. This coincides with severely reduced levels of 23S rRNA in pac leading to translational deficiencies and related alterations of plastid transcript patterns and abundance similar to plants treated with the translation inhibitor lincomycin. Thus, we conclude that deficiency in plastid ribosomes accounts for the pac phenotype. Moreover, the absence or reduction of PAC levels in the corresponding mutants induces structural changes of the 23S rRNA, as demonstrated by in vivo RNA structure probing. Our results indicate that PAC binds to the 23S rRNA to promote the biogenesis of the 50S subunit." @default.
- W2750082928 created "2017-08-31" @default.
- W2750082928 creator A5022745225 @default.
- W2750082928 creator A5056098534 @default.
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- W2750082928 creator A5067970331 @default.
- W2750082928 creator A5071208778 @default.
- W2750082928 creator A5087762478 @default.
- W2750082928 date "2017-09-21" @default.
- W2750082928 modified "2023-10-17" @default.
- W2750082928 title "PALE CRESS binds to plastid RNAs and facilitates the biogenesis of the 50S ribosomal subunit" @default.
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- W2750082928 doi "https://doi.org/10.1111/tpj.13662" @default.
- W2750082928 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28805278" @default.
- W2750082928 hasPublicationYear "2017" @default.
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