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• W2750236385 abstract "The ultimate goal of this research was to overcome the low immunogenicity of the biological macromolecule (heat stable enterotoxin STa) via its conjugation to biodegradable PLGA nanoparticles (NP). STa was first isolated from Enterotoxigenic Escherichia coli (ETEC), purified and identified using reported HPLC procedures. Optimized homogenous PLGA NP, prepared using the nanoprecipitation technique were used for conjugating STa using the carbodiimide synthesis. Covalent binding of STa to PLGA NP was confirmed via FTIR and 1HNMR analysis. Safety and tolerability of the developed nanoparticulated STa-PLGA conjugate were confirmed by MTT assay on A549 lung cancer cells. After subcutaneous immunization, STA-PLGA NP conjugate induced a significant immune response in mice showing a strong binding and neutralizing antibody titer. The developed novel STa-PLGA NP conjugate is expected to provide promising protection against enterotoxigenic Escherichia coli (ETEC)." @default.
• W2750236385 created "2017-08-31" @default.
• W2750236385 creator A5046140622 @default.
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• W2750236385 date "2018-01-01" @default.
• W2750236385 modified "2023-09-24" @default.
• W2750236385 title "Construction and immunogenicity analysis of nanoparticulated conjugate of heat-stable enterotoxin (STa) of enterotoxigenic Escherichia coli" @default.
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• W2750236385 doi "https://doi.org/10.1016/j.ijbiomac.2017.08.077" @default.
• W2750236385 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28823704" @default.
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