Matches in SemOpenAlex for { <https://semopenalex.org/work/W2750322906> ?p ?o ?g. }
- W2750322906 abstract "Abstract Across all domains of life, elaborate control mechanisms regulate proteins, pathways, and cell phenotypes as organisms adapt to ever-changing environments. Post-translational modifications (PTMs) allow cells to rapidly and reversibly regulate molecular pathways, but it remains unclear how individual PTMs regulate fitness. Here, we studied >130 PTM sites in Escherichia coli to unravel how PTMs regulate cell metabolism and fitness in response to environmental changes, such as the glucose-acetate diauxie. Using a new metabolic modeling approach, we found a significant fraction of post-translationally modified enzymes are predicted to control shifts in pathway usage following evolutionarily-important environmental changes. Genetic screens using Multiplex Automated Genome Engineering confirmed that these PTMs impact cellular fitness, especially under dynamically changing environments. Finally, mechanisms of how individual PTMs impact protein function were detailed using molecular dynamics simulations and enzyme assays for enolase, transaldolase, and serine hydroxymethyltransferase. Thus, by integrating whole-cell data and pathway modeling with detailed biochemical analysis, we unraveled how individual PTMs regulate enzymes, pathways, and phenotypes to adapt to sudden environmental changes." @default.
- W2750322906 created "2017-08-31" @default.
- W2750322906 creator A5002335463 @default.
- W2750322906 creator A5002596498 @default.
- W2750322906 creator A5002819499 @default.
- W2750322906 creator A5003459068 @default.
- W2750322906 creator A5013530299 @default.
- W2750322906 creator A5042031882 @default.
- W2750322906 creator A5045421762 @default.
- W2750322906 creator A5056560690 @default.
- W2750322906 creator A5058824230 @default.
- W2750322906 creator A5074929175 @default.
- W2750322906 creator A5078835630 @default.
- W2750322906 creator A5087457643 @default.
- W2750322906 date "2017-08-25" @default.
- W2750322906 modified "2023-10-10" @default.
- W2750322906 title "Systemic post-translational control of bacterial metabolism regulates adaptation in dynamic environments" @default.
- W2750322906 cites W1572613835 @default.
- W2750322906 cites W1871441147 @default.
- W2750322906 cites W1911678481 @default.
- W2750322906 cites W1966786968 @default.
- W2750322906 cites W1967039865 @default.
- W2750322906 cites W1969899640 @default.
- W2750322906 cites W1970103851 @default.
- W2750322906 cites W1972758026 @default.
- W2750322906 cites W1976161355 @default.
- W2750322906 cites W1981673913 @default.
- W2750322906 cites W1982309495 @default.
- W2750322906 cites W1988136571 @default.
- W2750322906 cites W1990122203 @default.
- W2750322906 cites W1994234835 @default.
- W2750322906 cites W2003153311 @default.
- W2750322906 cites W2004159979 @default.
- W2750322906 cites W2009163705 @default.
- W2750322906 cites W2011640015 @default.
- W2750322906 cites W2015292449 @default.
- W2750322906 cites W2015422619 @default.
- W2750322906 cites W2016995298 @default.
- W2750322906 cites W2017052832 @default.
- W2750322906 cites W2017196167 @default.
- W2750322906 cites W2019516978 @default.
- W2750322906 cites W2028979517 @default.
- W2750322906 cites W2031681107 @default.
- W2750322906 cites W2035199159 @default.
- W2750322906 cites W2035687084 @default.
- W2750322906 cites W2038758364 @default.
- W2750322906 cites W2044935306 @default.
- W2750322906 cites W2056333944 @default.
- W2750322906 cites W2056596773 @default.
- W2750322906 cites W2067174909 @default.
- W2750322906 cites W2067252347 @default.
- W2750322906 cites W2068855150 @default.
- W2750322906 cites W2068872007 @default.
- W2750322906 cites W2076165765 @default.
- W2750322906 cites W2076790509 @default.
- W2750322906 cites W2078355959 @default.
- W2750322906 cites W2082182783 @default.
- W2750322906 cites W2082382448 @default.
- W2750322906 cites W2083315745 @default.
- W2750322906 cites W2085482368 @default.
- W2750322906 cites W2089367187 @default.
- W2750322906 cites W2095020505 @default.
- W2750322906 cites W2098715150 @default.
- W2750322906 cites W2108078368 @default.
- W2750322906 cites W2111967267 @default.
- W2750322906 cites W2117474489 @default.
- W2750322906 cites W2120469353 @default.
- W2750322906 cites W2128776645 @default.
- W2750322906 cites W2131233236 @default.
- W2750322906 cites W2131288855 @default.
- W2750322906 cites W2133959349 @default.
- W2750322906 cites W2142270568 @default.
- W2750322906 cites W2144288821 @default.
- W2750322906 cites W2144779133 @default.
- W2750322906 cites W2147472054 @default.
- W2750322906 cites W2148557114 @default.
- W2750322906 cites W2149263899 @default.
- W2750322906 cites W2152186196 @default.
- W2750322906 cites W2152997175 @default.
- W2750322906 cites W2160541874 @default.
- W2750322906 cites W2165081628 @default.
- W2750322906 cites W2170427655 @default.
- W2750322906 cites W2313116527 @default.
- W2750322906 cites W2330799739 @default.
- W2750322906 cites W641516681 @default.
- W2750322906 doi "https://doi.org/10.1101/180646" @default.
- W2750322906 hasPublicationYear "2017" @default.
- W2750322906 type Work @default.
- W2750322906 sameAs 2750322906 @default.
- W2750322906 citedByCount "1" @default.
- W2750322906 countsByYear W27503229062018 @default.
- W2750322906 crossrefType "posted-content" @default.
- W2750322906 hasAuthorship W2750322906A5002335463 @default.
- W2750322906 hasAuthorship W2750322906A5002596498 @default.
- W2750322906 hasAuthorship W2750322906A5002819499 @default.
- W2750322906 hasAuthorship W2750322906A5003459068 @default.
- W2750322906 hasAuthorship W2750322906A5013530299 @default.
- W2750322906 hasAuthorship W2750322906A5042031882 @default.
- W2750322906 hasAuthorship W2750322906A5045421762 @default.
- W2750322906 hasAuthorship W2750322906A5056560690 @default.