FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2750608955> ?p ?o ?g. }

• W2750608955 endingPage "3365" @default.
• W2750608955 startingPage "3357" @default.
• W2750608955 abstract "The selection and dose of antibiotic therapy for biofilm-related infections are based on traditional pharmacokinetic studies using planktonic bacteria. The objective of this study was to characterize the time course and spatial activity of human exposure levels of meropenem and tobramycin against Pseudomonas aeruginosa biofilms grown in an in vitro flow-chamber model. Pharmacokinetic profiles of meropenem and tobramycin used in human therapy were administered to GFP-labelled P. aeruginosa PAO1 grown in flow chambers for 24 or 72 h. Images were acquired using confocal laser scanning microscopy throughout antibiotic treatment. Bacterial biomass was measured using COMSTAT and pharmacokinetic/pharmacodynamic models were fitted using NONMEM7. Meropenem treatment resulted in more rapid and sustained killing of both the 24 and 72 h PAO1 biofilm compared with tobramycin. Biofilm regrowth after antibiotic treatment occurred fastest with tobramycin. Meropenem preferentially killed subpopulations within the mushroom cap of the biofilms, regardless of biofilm maturity. The spatial killing by tobramycin varied with biofilm maturity. A tobramycin-treated 24 h biofilm resulted in live and dead cells detaching from the biofilm, while treatment of a 72 h biofilm preferentially killed subpopulations on the periphery of the mushroom stalk. Regrowth occurred primarily on the mushroom caps. Combination meropenem and tobramycin therapy resulted in rapid and efficient killing of biofilm cells, with a spatial pattern similar to meropenem alone. Simulated human concentrations of meropenem and tobramycin in young and mature PAO1 biofilms exhibited differences in temporal and spatial patterns of killing and antibiotic tolerance development." @default.
• W2750608955 created "2017-09-15" @default.
• W2750608955 creator A5003818696 @default.
• W2750608955 creator A5037861831 @default.
• W2750608955 creator A5038545801 @default.
• W2750608955 creator A5046285994 @default.
• W2750608955 creator A5076124051 @default.
• W2750608955 creator A5084251510 @default.
• W2750608955 date "2017-09-06" @default.
• W2750608955 modified "2023-10-18" @default.
• W2750608955 title "Spatiotemporal pharmacodynamics of meropenem- and tobramycin-treated Pseudomonas aeruginosa biofilms" @default.
• W2750608955 cites W1534758791 @default.
• W2750608955 cites W1543474898 @default.
• W2750608955 cites W1966822490 @default.
• W2750608955 cites W1968544058 @default.
• W2750608955 cites W1971239627 @default.
• W2750608955 cites W1974637030 @default.
• W2750608955 cites W1974788123 @default.
• W2750608955 cites W1990024058 @default.
• W2750608955 cites W1991811123 @default.
• W2750608955 cites W2015984063 @default.
• W2750608955 cites W2018874852 @default.
• W2750608955 cites W2021613721 @default.
• W2750608955 cites W2021639750 @default.
• W2750608955 cites W2025801951 @default.
• W2750608955 cites W2042237163 @default.
• W2750608955 cites W2054878069 @default.
• W2750608955 cites W2067756304 @default.
• W2750608955 cites W2069549031 @default.
• W2750608955 cites W2078681628 @default.
• W2750608955 cites W2080936059 @default.
• W2750608955 cites W2097872664 @default.
• W2750608955 cites W2100285085 @default.
• W2750608955 cites W2102019441 @default.
• W2750608955 cites W2103884572 @default.
• W2750608955 cites W2106351298 @default.
• W2750608955 cites W2114510377 @default.
• W2750608955 cites W2117881268 @default.
• W2750608955 cites W2119608865 @default.
• W2750608955 cites W2122094987 @default.
• W2750608955 cites W2122608498 @default.
• W2750608955 cites W2126221953 @default.
• W2750608955 cites W2132504853 @default.
• W2750608955 cites W2132927585 @default.
• W2750608955 cites W2134515577 @default.
• W2750608955 cites W2138779519 @default.
• W2750608955 cites W2138904898 @default.
• W2750608955 cites W2142635246 @default.
• W2750608955 cites W2150927592 @default.
• W2750608955 cites W2152761448 @default.
• W2750608955 cites W2156495539 @default.
• W2750608955 cites W2156731012 @default.
• W2750608955 cites W2156920884 @default.
• W2750608955 cites W2158350453 @default.
• W2750608955 cites W2162335168 @default.
• W2750608955 cites W2168175751 @default.
• W2750608955 cites W2171039477 @default.
• W2750608955 cites W2341166238 @default.
• W2750608955 cites W2403357711 @default.
• W2750608955 doi "https://doi.org/10.1093/jac/dkx288" @default.
• W2750608955 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5890705" @default.
• W2750608955 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28961810" @default.
• W2750608955 hasPublicationYear "2017" @default.
• W2750608955 type Work @default.
• W2750608955 sameAs 2750608955 @default.
• W2750608955 citedByCount "23" @default.
• W2750608955 countsByYear W27506089552018 @default.
• W2750608955 countsByYear W27506089552019 @default.
• W2750608955 countsByYear W27506089552020 @default.
• W2750608955 countsByYear W27506089552021 @default.
• W2750608955 countsByYear W27506089552022 @default.
• W2750608955 countsByYear W27506089552023 @default.
• W2750608955 crossrefType "journal-article" @default.
• W2750608955 hasAuthorship W2750608955A5003818696 @default.
• W2750608955 hasAuthorship W2750608955A5037861831 @default.
• W2750608955 hasAuthorship W2750608955A5038545801 @default.
• W2750608955 hasAuthorship W2750608955A5046285994 @default.
• W2750608955 hasAuthorship W2750608955A5076124051 @default.
• W2750608955 hasAuthorship W2750608955A5084251510 @default.
• W2750608955 hasBestOaLocation W27506089551 @default.
• W2750608955 hasConcept C111113717 @default.
• W2750608955 hasConcept C112705442 @default.
• W2750608955 hasConcept C185592680 @default.
• W2750608955 hasConcept C2775832221 @default.
• W2750608955 hasConcept C2777637488 @default.
• W2750608955 hasConcept C2778974779 @default.
• W2750608955 hasConcept C2779375183 @default.
• W2750608955 hasConcept C501593827 @default.
• W2750608955 hasConcept C523546767 @default.
• W2750608955 hasConcept C54355233 @default.
• W2750608955 hasConcept C58123911 @default.
• W2750608955 hasConcept C86803240 @default.
• W2750608955 hasConcept C89423630 @default.
• W2750608955 hasConcept C94665300 @default.
• W2750608955 hasConcept C98274493 @default.
• W2750608955 hasConceptScore W2750608955C111113717 @default.
• W2750608955 hasConceptScore W2750608955C112705442 @default.
• W2750608955 hasConceptScore W2750608955C185592680 @default.

• next