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• W2750904889 endingPage "2943" @default.
• W2750904889 startingPage "2927" @default.
• W2750904889 abstract "Efficient production of ethanol for use as a renewable fuel requires organisms with a high level of ethanol tolerance. However, this trait is complex and increased tolerance therefore requires mutations in multiple genes and pathways. Here, we use experimental evolution for a system-level analysis of adaptation of Escherichia coli to high ethanol stress. As adaptation to extreme stress often results in complex mutational data sets consisting of both causal and noncausal passenger mutations, identifying the true adaptive mutations in these settings is not trivial. Therefore, we developed a novel method named IAMBEE (Identification of Adaptive Mutations in Bacterial Evolution Experiments). IAMBEE exploits the temporal profile of the acquisition of mutations during evolution in combination with the functional implications of each mutation at the protein level. These data are mapped to a genome-wide interaction network to search for adaptive mutations at the level of pathways. The 16 evolved populations in our data set together harbored 2,286 mutated genes with 4,470 unique mutations. Analysis by IAMBEE significantly reduced this number and resulted in identification of 90 mutated genes and 345 unique mutations that are most likely to be adaptive. Moreover, IAMBEE not only enabled the identification of previously known pathways involved in ethanol tolerance, but also identified novel systems such as the AcrAB-TolC efflux pump and fatty acids biosynthesis and even allowed to gain insight into the temporal profile of adaptation to ethanol stress. Furthermore, this method offers a solid framework for identifying the molecular underpinnings of other complex traits as well." @default.
• W2750904889 created "2017-09-15" @default.
• W2750904889 creator A5005685888 @default.
• W2750904889 creator A5010819806 @default.
• W2750904889 creator A5044805939 @default.
• W2750904889 creator A5047309373 @default.
• W2750904889 creator A5063661741 @default.
• W2750904889 creator A5079221065 @default.
• W2750904889 creator A5079297835 @default.
• W2750904889 date "2017-08-28" @default.
• W2750904889 modified "2023-10-12" @default.
• W2750904889 title "Network-Based Identification of Adaptive Pathways in Evolved Ethanol-Tolerant Bacterial Populations" @default.
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• W2750904889 doi "https://doi.org/10.1093/molbev/msx228" @default.

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