Matches in SemOpenAlex for { <https://semopenalex.org/work/W2750991179> ?p ?o ?g. }
Showing items 1 to 62 of
62
with 100 items per page.
- W2750991179 abstract "Purpose Human choroidal melanocytes ( HCM s) are an abundant heterogeneous, melanin‐containing population of cells within the vascular uveal tract. Toll‐like receptors ( TLR s) are pattern recognition receptors involved in the innate immune response against phylogenetically conserved microbial components. Immune cells are established to express TLR s and more recent studies showed TLR expression on non‐immune cells including corneal and conjunctival epithelia, retinal microglia and RPE , iris pigment epithelium, and skin melanocytes. The expression of TLR s on uveal melanocytes however remains to be investigated. Methods HCM s were isolated and cultured from donor human eyes. TLR 1‐10 and MYD 88 (a critical adaptor protein in the TLR signalling pathway) expression was investigated using RT ‐ PCR and confirmed by immunocytochemistry. The production of IL ‐8 and CCL 2 in response to TLR agonists was measured by ELISA . Results HCM s constitutively expressed TLR 1‐6, TLR 9, and MYD 88. Stimulation of HCM s with bacterial‐derived TLR agonists [Pam3 CSK 4 ( TLR 1/2), HKLM ( TLR 2), LPS ( TLR 4), Flagellin ( TLR 5) and, FSL ‐1 ( TLR 6/2)] and virus‐mimicking TLR agonists [Poly I:C ( TLR 3), Imiquimod ( TLR 7), ss RNA 40 ( TLR 8) and ODN 2006 ( TLR 9)] induced variable secretion of IL ‐8 and CCL 2 ( MCP ‐1). Control levels of IL 8 (140 pg/ml) increased in response to Pam3 CSK 4 (2360 pg/ml), Poly I:C (3620 pg/ml), LPS (4100 pg/ml), Flagellin (1550 pg/ml) and FSL ‐1 (2160 pg/ml). Virus‐mimicking agonists imiquimod, ss RNA 40 and ODN 2006, and intriguingly bacteria‐derived HKML , did not significantly affect secreted IL 8. Secreted CCL 2 showed a similar pattern of response following stimulation with TLR agonists. HCM s were immunoreactive for TLR 2, TLR 3 and TLR 4 protein. Conclusions The findings suggest that HCM s are potentially involved in innate immune responses against bacterial and viral pathogens within the human choroid." @default.
- W2750991179 created "2017-09-15" @default.
- W2750991179 creator A5004003955 @default.
- W2750991179 creator A5027161649 @default.
- W2750991179 creator A5039478629 @default.
- W2750991179 date "2017-09-01" @default.
- W2750991179 modified "2023-09-23" @default.
- W2750991179 title "Primary human choroidal melanocytes express functional Toll-Like Receptors (TLRs)" @default.
- W2750991179 doi "https://doi.org/10.1111/j.1755-3768.2017.03346" @default.
- W2750991179 hasPublicationYear "2017" @default.
- W2750991179 type Work @default.
- W2750991179 sameAs 2750991179 @default.
- W2750991179 citedByCount "1" @default.
- W2750991179 countsByYear W27509911792018 @default.
- W2750991179 crossrefType "journal-article" @default.
- W2750991179 hasAuthorship W2750991179A5004003955 @default.
- W2750991179 hasAuthorship W2750991179A5027161649 @default.
- W2750991179 hasAuthorship W2750991179A5039478629 @default.
- W2750991179 hasBestOaLocation W27509911791 @default.
- W2750991179 hasConcept C136449434 @default.
- W2750991179 hasConcept C153911025 @default.
- W2750991179 hasConcept C170493617 @default.
- W2750991179 hasConcept C185592680 @default.
- W2750991179 hasConcept C203014093 @default.
- W2750991179 hasConcept C2776709828 @default.
- W2750991179 hasConcept C2778420691 @default.
- W2750991179 hasConcept C38117629 @default.
- W2750991179 hasConcept C55493867 @default.
- W2750991179 hasConcept C86803240 @default.
- W2750991179 hasConcept C8891405 @default.
- W2750991179 hasConcept C95444343 @default.
- W2750991179 hasConceptScore W2750991179C136449434 @default.
- W2750991179 hasConceptScore W2750991179C153911025 @default.
- W2750991179 hasConceptScore W2750991179C170493617 @default.
- W2750991179 hasConceptScore W2750991179C185592680 @default.
- W2750991179 hasConceptScore W2750991179C203014093 @default.
- W2750991179 hasConceptScore W2750991179C2776709828 @default.
- W2750991179 hasConceptScore W2750991179C2778420691 @default.
- W2750991179 hasConceptScore W2750991179C38117629 @default.
- W2750991179 hasConceptScore W2750991179C55493867 @default.
- W2750991179 hasConceptScore W2750991179C86803240 @default.
- W2750991179 hasConceptScore W2750991179C8891405 @default.
- W2750991179 hasConceptScore W2750991179C95444343 @default.
- W2750991179 hasIssue "S259" @default.
- W2750991179 hasLocation W27509911791 @default.
- W2750991179 hasOpenAccess W2750991179 @default.
- W2750991179 hasPrimaryLocation W27509911791 @default.
- W2750991179 hasRelatedWork W1519633296 @default.
- W2750991179 hasRelatedWork W1931231627 @default.
- W2750991179 hasRelatedWork W2084402420 @default.
- W2750991179 hasRelatedWork W2107182434 @default.
- W2750991179 hasRelatedWork W2126834509 @default.
- W2750991179 hasRelatedWork W2141330728 @default.
- W2750991179 hasRelatedWork W2361051996 @default.
- W2750991179 hasRelatedWork W2792679014 @default.
- W2750991179 hasRelatedWork W3200993532 @default.
- W2750991179 hasRelatedWork W4323348676 @default.
- W2750991179 hasVolume "95" @default.
- W2750991179 isParatext "false" @default.
- W2750991179 isRetracted "false" @default.
- W2750991179 magId "2750991179" @default.
- W2750991179 workType "article" @default.