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- W2751023815 abstract "Gene silencing using small interfering ribonucleic acids (siRNA) is a powerful method to interfere with gene expression, allowing for the functional exploration of specific genes. siRNA interference can be applied in both cell lines, as well as in primary, non-dividing cell types like dendritic cells. However, the efficacy in different cell types is variable and requires optimization. Here, we showed that the type of culture medium used during lipid-based siRNA-mediated transfection acts as a critical factor, affecting dendritic cell activation. Transfection of immature monocyte-derived dendritic cells in RPMI medium, but not in IMDM, showed increased transcript levels of pro-inflammatory cytokines. Moreover, the expression of co-stimulatory molecules was enhanced, thereby increasing the T cell stimulatory capacity. Our data demonstrates that the choice of medium should be critically examined as one of the variables while optimizing cell transfection." @default.
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- W2751023815 date "2017-09-01" @default.
- W2751023815 modified "2023-09-26" @default.
- W2751023815 title "CD40 ligation on antigen presenting cells (APCs) enhances properdin production" @default.
- W2751023815 doi "https://doi.org/10.1016/j.molimm.2017.06.066" @default.
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