Matches in SemOpenAlex for { <https://semopenalex.org/work/W2751266054> ?p ?o ?g. }
Showing items 1 to 80 of
80
with 100 items per page.
- W2751266054 abstract "Aim To prevent serious problem due to cytomegalovirus (CMV) infection, antiviral prophylaxis is commonly used in recipients of solid-organ transplants. To predict which patient will develop CMV disease, CMV-specific cell-mediated immunity can be measured by Quantiferon-CMV, ELISpot assay, cytokine profiling, intracellular cytokine staining and tetramer staining. We were comparing Quantiferon-CMV and ELISpot assay in the recipient of liver transplantation in Korea, in which most people are seropositive. Methods We prospectively included 35 patients who will receive antiviral prophylaxis for liver transplantation in a tertiary university hospital. We had tested interferon-gamma level following in vitro stimulation with CMV antigen using two assays – EliSpot IFN-γ CMVspot (CMVspot) (Autoimmun Diagnostika, Germany) and Quantiferon-CMV (QF-CMV) (Qiagen, Australia). Blood samples of recipient collected before liver transplantation and CMVspot, CF-CMV and CMV IgM/IgG assay (BioMerieux, Germany) were performed simultaneously. For detection of CMV viremia, real-time PCR was performed using CMV R-gene kit (BioMerieux, France). The testing and interpretation were performed according to the manufacturer’s recommendation. Results The agreement rate of two assays was overall 51.5%. Although CMVspot results showed 32 recipients has CMV-specific cell-mediated immunity, 17 patients show reactive results in the QF-CMV assay. The 28 patients show the reactive result of CMVspot pp65 and the 13 patients show reactive results of CMVspot IE-1. Two samples were failing during the process of CMVspot on the sample reception and interpretation step and seven samples were interpreted indeterminate because of low mitogen reactivity in QF-CMV. All patients had a positive result of CMV IgG before transplantation and 16 patients had CMV viremia episode after transplantation. Conclusions To choose the method for testing cell-mediated immunity for CMV, laboratory have to concern about the characteristics of assays and regional seropositive prevalence. The results of two commercial assays can be different at one time for testing. Further evaluation was proceeding for accurate prediction of CMV disease after solid-organ transplantation." @default.
- W2751266054 created "2017-09-15" @default.
- W2751266054 creator A5012376271 @default.
- W2751266054 creator A5040708060 @default.
- W2751266054 creator A5057383019 @default.
- W2751266054 creator A5078133001 @default.
- W2751266054 creator A5091385586 @default.
- W2751266054 date "2017-09-01" @default.
- W2751266054 modified "2023-09-27" @default.
- W2751266054 title "P208 Comparison of two assays for CMV specific cell mediated immunity in the recipients of liver transplantation" @default.
- W2751266054 doi "https://doi.org/10.1016/j.humimm.2017.06.268" @default.
- W2751266054 hasPublicationYear "2017" @default.
- W2751266054 type Work @default.
- W2751266054 sameAs 2751266054 @default.
- W2751266054 citedByCount "0" @default.
- W2751266054 crossrefType "journal-article" @default.
- W2751266054 hasAuthorship W2751266054A5012376271 @default.
- W2751266054 hasAuthorship W2751266054A5040708060 @default.
- W2751266054 hasAuthorship W2751266054A5057383019 @default.
- W2751266054 hasAuthorship W2751266054A5078133001 @default.
- W2751266054 hasAuthorship W2751266054A5091385586 @default.
- W2751266054 hasConcept C126322002 @default.
- W2751266054 hasConcept C159047783 @default.
- W2751266054 hasConcept C159654299 @default.
- W2751266054 hasConcept C203014093 @default.
- W2751266054 hasConcept C2522874641 @default.
- W2751266054 hasConcept C2776090121 @default.
- W2751266054 hasConcept C2776185481 @default.
- W2751266054 hasConcept C2776409557 @default.
- W2751266054 hasConcept C2777732132 @default.
- W2751266054 hasConcept C2779053233 @default.
- W2751266054 hasConcept C2779609443 @default.
- W2751266054 hasConcept C2780727368 @default.
- W2751266054 hasConcept C2911091166 @default.
- W2751266054 hasConcept C3017875465 @default.
- W2751266054 hasConcept C71924100 @default.
- W2751266054 hasConcept C8891405 @default.
- W2751266054 hasConceptScore W2751266054C126322002 @default.
- W2751266054 hasConceptScore W2751266054C159047783 @default.
- W2751266054 hasConceptScore W2751266054C159654299 @default.
- W2751266054 hasConceptScore W2751266054C203014093 @default.
- W2751266054 hasConceptScore W2751266054C2522874641 @default.
- W2751266054 hasConceptScore W2751266054C2776090121 @default.
- W2751266054 hasConceptScore W2751266054C2776185481 @default.
- W2751266054 hasConceptScore W2751266054C2776409557 @default.
- W2751266054 hasConceptScore W2751266054C2777732132 @default.
- W2751266054 hasConceptScore W2751266054C2779053233 @default.
- W2751266054 hasConceptScore W2751266054C2779609443 @default.
- W2751266054 hasConceptScore W2751266054C2780727368 @default.
- W2751266054 hasConceptScore W2751266054C2911091166 @default.
- W2751266054 hasConceptScore W2751266054C3017875465 @default.
- W2751266054 hasConceptScore W2751266054C71924100 @default.
- W2751266054 hasConceptScore W2751266054C8891405 @default.
- W2751266054 hasLocation W27512660541 @default.
- W2751266054 hasOpenAccess W2751266054 @default.
- W2751266054 hasPrimaryLocation W27512660541 @default.
- W2751266054 hasRelatedWork W1966893343 @default.
- W2751266054 hasRelatedWork W2116796568 @default.
- W2751266054 hasRelatedWork W2118597625 @default.
- W2751266054 hasRelatedWork W2151265214 @default.
- W2751266054 hasRelatedWork W2281644518 @default.
- W2751266054 hasRelatedWork W2289004520 @default.
- W2751266054 hasRelatedWork W2368750230 @default.
- W2751266054 hasRelatedWork W2371796487 @default.
- W2751266054 hasRelatedWork W2409608732 @default.
- W2751266054 hasRelatedWork W2472648835 @default.
- W2751266054 hasRelatedWork W2765752136 @default.
- W2751266054 hasRelatedWork W2771091474 @default.
- W2751266054 hasRelatedWork W2806957451 @default.
- W2751266054 hasRelatedWork W2923466622 @default.
- W2751266054 hasRelatedWork W2971395244 @default.
- W2751266054 hasRelatedWork W3020559384 @default.
- W2751266054 hasRelatedWork W3034077774 @default.
- W2751266054 hasRelatedWork W3049547631 @default.
- W2751266054 hasRelatedWork W3123831789 @default.
- W2751266054 hasRelatedWork W38134607 @default.
- W2751266054 isParatext "false" @default.
- W2751266054 isRetracted "false" @default.
- W2751266054 magId "2751266054" @default.
- W2751266054 workType "article" @default.