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- W2751308098 abstract "To study the replication and antiviral innate immunity of EV-A71 in mouse macrophages, we selected the mouse macrophage RAW264.7cell line as a model. An absolute quantitative PCR detection method was constructed to detect the viral load of EV-A71 in RAW264.7cells.RT-qPCR detected the fold changes of the proinflammatory cytokine, chemokine, and pattern recognition receptors at different time points post-infection in RAW264.7cells infected with EV-A71 and UV-inactivated EV-A71.The results revealed that the viral load of EV-A71 in RAW264.7cells decreased as the time post-infection increased. Proinflammatory cytokines, including IL-1β,IL-6,TNF-α,and chemokines, including IP-10,MCP-1,and MIP-1αwere induced, and the mRNA expression levels of TLR2,TLR1,TLR6,MDA5,and RIG-I were up-regulated. These results indicate that EV-A71 could replicate in mouse macrophages at a lower level, and proinflammatory cytokine and chemokine responses were induced." @default.
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- W2751308098 date "2016-05-01" @default.
- W2751308098 modified "2023-09-22" @default.
- W2751308098 title "[Proinflammatory Cytokine and Chemokine Production of Mouse Macrophages in Response to EV-A71 Infection]." @default.
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