Matches in SemOpenAlex for { <https://semopenalex.org/work/W2751535887> ?p ?o ?g. }
- W2751535887 endingPage "457" @default.
- W2751535887 startingPage "433" @default.
- W2751535887 abstract "The breaking of RNA strands by 2′-O-transphosphorylation is a ubiquitous reaction in biology, and enzymes that catalyze this reaction play key roles in RNA metabolism. The mechanisms of 2′-O-transphosphorylation in solution are relatively well studied, but complex and can involve different transition states depending on how the reaction is catalyzed. Because of this complexity and the lack of experimental information on transition-state structure, pinning down the chemical details of enzyme-catalyzed RNA strand cleavage has been difficult. Kinetic isotope effects (KIEs) provide information about changes in bonding as a reaction proceeds from ground state to transition state, and therefore they provide a powerful tool for revealing mechanistic detail. Application of kinetic isotope analyses to RNA 2′-O-transphosphorylation faces three fundamental challenges: synthesis of RNA substrate isotopomers with 18O substitutions at the 2′-O, 5′-O and nonbridging phosphoryl oxygens; determination of the 18O/16O ratios in the residual unreacted substrate or product RNAs; and analyzing these data to allow calculation of the KIEs for use in evaluating different mechanistic scenarios. In this chapter, we outline methods for surmounting these challenges for solution RNA 2′-O-transphosphorylation reactions, and we describe their initial application to understand nonenzymatic solution reactions and reactions catalyzed by the enzyme ribonuclease A." @default.
- W2751535887 created "2017-09-15" @default.
- W2751535887 creator A5012071618 @default.
- W2751535887 creator A5012692916 @default.
- W2751535887 creator A5076436548 @default.
- W2751535887 creator A5086859418 @default.
- W2751535887 date "2017-01-01" @default.
- W2751535887 modified "2023-09-25" @default.
- W2751535887 title "Kinetic Isotope Effect Analysis of RNA 2′- O -Transphosphorylation" @default.
- W2751535887 cites W1522250660 @default.
- W2751535887 cites W1535452251 @default.
- W2751535887 cites W1571253423 @default.
- W2751535887 cites W1607420008 @default.
- W2751535887 cites W1825277304 @default.
- W2751535887 cites W1953904050 @default.
- W2751535887 cites W1967018847 @default.
- W2751535887 cites W1968580578 @default.
- W2751535887 cites W1970961719 @default.
- W2751535887 cites W1971589546 @default.
- W2751535887 cites W1975497571 @default.
- W2751535887 cites W1979034187 @default.
- W2751535887 cites W1985023196 @default.
- W2751535887 cites W2004815619 @default.
- W2751535887 cites W2006207911 @default.
- W2751535887 cites W2007432361 @default.
- W2751535887 cites W2012551031 @default.
- W2751535887 cites W2015293171 @default.
- W2751535887 cites W2017886632 @default.
- W2751535887 cites W2020677628 @default.
- W2751535887 cites W2026524671 @default.
- W2751535887 cites W2041998286 @default.
- W2751535887 cites W2045837783 @default.
- W2751535887 cites W2051088843 @default.
- W2751535887 cites W2051282925 @default.
- W2751535887 cites W2054212268 @default.
- W2751535887 cites W2057923138 @default.
- W2751535887 cites W2058762498 @default.
- W2751535887 cites W2062339554 @default.
- W2751535887 cites W2063210086 @default.
- W2751535887 cites W2072192872 @default.
- W2751535887 cites W2072245156 @default.
- W2751535887 cites W2075769366 @default.
- W2751535887 cites W2076527540 @default.
- W2751535887 cites W2076926610 @default.
- W2751535887 cites W2085716962 @default.
- W2751535887 cites W2087629026 @default.
- W2751535887 cites W2088659205 @default.
- W2751535887 cites W2091088143 @default.
- W2751535887 cites W2093860461 @default.
- W2751535887 cites W2099291613 @default.
- W2751535887 cites W2104951635 @default.
- W2751535887 cites W2113568083 @default.
- W2751535887 cites W2113627793 @default.
- W2751535887 cites W2154378035 @default.
- W2751535887 cites W2159421412 @default.
- W2751535887 cites W2161812240 @default.
- W2751535887 cites W2163306024 @default.
- W2751535887 cites W2194725007 @default.
- W2751535887 cites W2325320324 @default.
- W2751535887 cites W2331416944 @default.
- W2751535887 cites W2607451541 @default.
- W2751535887 cites W2625154988 @default.
- W2751535887 cites W965565424 @default.
- W2751535887 doi "https://doi.org/10.1016/bs.mie.2017.07.017" @default.
- W2751535887 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28911780" @default.
- W2751535887 hasPublicationYear "2017" @default.
- W2751535887 type Work @default.
- W2751535887 sameAs 2751535887 @default.
- W2751535887 citedByCount "3" @default.
- W2751535887 countsByYear W27515358872018 @default.
- W2751535887 countsByYear W27515358872021 @default.
- W2751535887 countsByYear W27515358872023 @default.
- W2751535887 crossrefType "book-chapter" @default.
- W2751535887 hasAuthorship W2751535887A5012071618 @default.
- W2751535887 hasAuthorship W2751535887A5012692916 @default.
- W2751535887 hasAuthorship W2751535887A5076436548 @default.
- W2751535887 hasAuthorship W2751535887A5086859418 @default.
- W2751535887 hasConcept C104317684 @default.
- W2751535887 hasConcept C121332964 @default.
- W2751535887 hasConcept C127124265 @default.
- W2751535887 hasConcept C147597530 @default.
- W2751535887 hasConcept C178790620 @default.
- W2751535887 hasConcept C181199279 @default.
- W2751535887 hasConcept C185592680 @default.
- W2751535887 hasConcept C18903297 @default.
- W2751535887 hasConcept C2777289219 @default.
- W2751535887 hasConcept C32909587 @default.
- W2751535887 hasConcept C55493867 @default.
- W2751535887 hasConcept C58364064 @default.
- W2751535887 hasConcept C62520636 @default.
- W2751535887 hasConcept C67705224 @default.
- W2751535887 hasConcept C69928629 @default.
- W2751535887 hasConcept C71240020 @default.
- W2751535887 hasConcept C86803240 @default.
- W2751535887 hasConceptScore W2751535887C104317684 @default.
- W2751535887 hasConceptScore W2751535887C121332964 @default.
- W2751535887 hasConceptScore W2751535887C127124265 @default.
- W2751535887 hasConceptScore W2751535887C147597530 @default.