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- W2751561700 abstract "Flat mounting of the whole cornea has two main advantages over conventional cross sections: it provides an overview of the whole tissue while allowing precise subcellular localization. We first described how to improve immunostaining for the analysis of the endothelial cells of flat-mounted corneas, by using fixation with methanol or 0.5% PFA at room temperature, and not the conventional fixation with 4% PFA. We then applied a similar technique for the multilayered epithelium. Flat mounting is essential to study the distribution of rare cells inside large area of cells." @default.
- W2751561700 created "2017-09-15" @default.
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- W2751561700 date "2017-09-01" @default.
- W2751561700 modified "2023-09-26" @default.
- W2751561700 title "Flat-mount preparation of cornea" @default.
- W2751561700 doi "https://doi.org/10.1111/j.1755-3768.2017.01341" @default.
- W2751561700 hasPublicationYear "2017" @default.
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