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- W2751646908 abstract "γ-Tubulin has a well-established role in nucleating the assembly of microtubules, yet how phosphorylation regulates its activity remains unclear. Here, we use a time-resolved, fitness-based SGA approach to compare two γ-tubulin alleles, and find that the genetic interaction profile of γtub-Y362E is enriched in spindle positioning and cell polarity genes relative to that of γtub-Y445D, which is enriched in genes involved in spindle assembly and stability. In γtub-Y362E cells, we find a defect in spindle alignment and an increase in the number of astral microtubules at both spindle poles. Our results suggest that the γtub-Y362E allele is a separation-of-function mutation that reveals a role for γ-tubulin phospho-regulation in spindle alignment. We propose that phosphorylation of the evolutionarily conserved Y362 residue of budding yeast γ-tubulin contributes to regulating the number of astral microtubules associated with spindle poles, and promoting efficient pre-anaphase spindle alignment." @default.
- W2751646908 created "2017-09-15" @default.
- W2751646908 creator A5005284140 @default.
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- W2751646908 date "2017-09-12" @default.
- W2751646908 modified "2023-10-16" @default.
- W2751646908 title "Interrogation of γ-tubulin alleles using high-resolution fitness measurements reveals a distinct cytoplasmic function in spindle alignment" @default.
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- W2751646908 doi "https://doi.org/10.1038/s41598-017-11789-7" @default.
- W2751646908 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5595808" @default.
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- W2751646908 hasPublicationYear "2017" @default.
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