Matches in SemOpenAlex for { <https://semopenalex.org/work/W2751928832> ?p ?o ?g. }
- W2751928832 abstract "How position-dependent cell fate acquisition occurs during embryogenesis is a central question in developmental biology. To study this process, we developed a defined, high-throughput assay to induce peri-gastrulation-associated patterning in geometrically-confined human pluripotent stem cell (hPSC) colonies. We observed that, upon BMP4 treatment, phosphorylated SMAD1 (pSMAD1) activity in the colonies organized into a radial gradient. We developed a Reaction-Diffusion (RD) based computational model and observed that the self-organization of pSMAD1 signaling was consistent with the RD principle. Consequent fate acquisition occurred as a function of both pSMAD1 signaling strength and duration of induction – consistent with the Positional-Information (PI) paradigm. We propose that the self-organized peri-gastrulation-like fate patterning in BMP4 treated geometrically-confined hPSC colonies arises via a stepwise model of RD, and PI. This two-step model predicted experimental responses to perturbations of key parameters such as colony size, and BMP4 dose. Furthermore, it also predicted experimental conditions that resulted in RD-like periodic patterning in large hPSC colonies, and rescued peri-gastrulation-like patterning in colony sizes previously thought to be reticent to this behaviour." @default.
- W2751928832 created "2017-09-15" @default.
- W2751928832 creator A5014416721 @default.
- W2751928832 creator A5026092817 @default.
- W2751928832 creator A5045565587 @default.
- W2751928832 creator A5051668549 @default.
- W2751928832 creator A5052129929 @default.
- W2751928832 creator A5078934880 @default.
- W2751928832 creator A5083965334 @default.
- W2751928832 date "2017-01-01" @default.
- W2751928832 modified "2023-10-18" @default.
- W2751928832 title "A stepwise model of Reaction-Diffusion and Positional-Information governs self-organized human peri-gastrulation-like patterning" @default.
- W2751928832 cites W1887465480 @default.
- W2751928832 cites W1969357685 @default.
- W2751928832 cites W1970083872 @default.
- W2751928832 cites W1970147973 @default.
- W2751928832 cites W1974210089 @default.
- W2751928832 cites W1977509897 @default.
- W2751928832 cites W1977858547 @default.
- W2751928832 cites W1978716417 @default.
- W2751928832 cites W1980661496 @default.
- W2751928832 cites W1988397986 @default.
- W2751928832 cites W1989874642 @default.
- W2751928832 cites W1990938412 @default.
- W2751928832 cites W1991138583 @default.
- W2751928832 cites W1994525772 @default.
- W2751928832 cites W1995706513 @default.
- W2751928832 cites W1996709598 @default.
- W2751928832 cites W1998317358 @default.
- W2751928832 cites W2003095799 @default.
- W2751928832 cites W2003211588 @default.
- W2751928832 cites W2012793556 @default.
- W2751928832 cites W2013438278 @default.
- W2751928832 cites W2016350172 @default.
- W2751928832 cites W2017381676 @default.
- W2751928832 cites W2018744859 @default.
- W2751928832 cites W2024181148 @default.
- W2751928832 cites W2030093272 @default.
- W2751928832 cites W2031811488 @default.
- W2751928832 cites W2031928671 @default.
- W2751928832 cites W2033059921 @default.
- W2751928832 cites W2034609545 @default.
- W2751928832 cites W2036552662 @default.
- W2751928832 cites W2037486322 @default.
- W2751928832 cites W2039888453 @default.
- W2751928832 cites W2043085906 @default.
- W2751928832 cites W2044193480 @default.
- W2751928832 cites W2050351042 @default.
- W2751928832 cites W2054132545 @default.
- W2751928832 cites W2056089399 @default.
- W2751928832 cites W2056298328 @default.
- W2751928832 cites W2059145054 @default.
- W2751928832 cites W2059383089 @default.
- W2751928832 cites W2063249111 @default.
- W2751928832 cites W2069693786 @default.
- W2751928832 cites W2073881036 @default.
- W2751928832 cites W2075994912 @default.
- W2751928832 cites W2076812266 @default.
- W2751928832 cites W2077765163 @default.
- W2751928832 cites W2079331738 @default.
- W2751928832 cites W2082152178 @default.
- W2751928832 cites W2083249373 @default.
- W2751928832 cites W2085543001 @default.
- W2751928832 cites W2089840306 @default.
- W2751928832 cites W2091643574 @default.
- W2751928832 cites W2091699754 @default.
- W2751928832 cites W2092099264 @default.
- W2751928832 cites W2093527972 @default.
- W2751928832 cites W2095762981 @default.
- W2751928832 cites W2101784587 @default.
- W2751928832 cites W2107307011 @default.
- W2751928832 cites W2117814548 @default.
- W2751928832 cites W2126664377 @default.
- W2751928832 cites W2128393954 @default.
- W2751928832 cites W2132581785 @default.
- W2751928832 cites W2135447402 @default.
- W2751928832 cites W2140909735 @default.
- W2751928832 cites W2143368326 @default.
- W2751928832 cites W2171109270 @default.
- W2751928832 cites W2207941023 @default.
- W2751928832 cites W2261586944 @default.
- W2751928832 cites W2397191557 @default.
- W2751928832 cites W2461230718 @default.
- W2751928832 cites W2531559910 @default.
- W2751928832 cites W2535856472 @default.
- W2751928832 cites W2618403165 @default.
- W2751928832 cites W2622190705 @default.
- W2751928832 cites W2991126964 @default.
- W2751928832 doi "https://doi.org/10.1242/dev.149658" @default.
- W2751928832 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5769627" @default.
- W2751928832 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28870989" @default.
- W2751928832 hasPublicationYear "2017" @default.
- W2751928832 type Work @default.
- W2751928832 sameAs 2751928832 @default.
- W2751928832 citedByCount "119" @default.
- W2751928832 countsByYear W27519288322017 @default.
- W2751928832 countsByYear W27519288322018 @default.
- W2751928832 countsByYear W27519288322019 @default.
- W2751928832 countsByYear W27519288322020 @default.
- W2751928832 countsByYear W27519288322021 @default.