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• W2751976941 abstract "The proper formation of synapses-specialized unitary structures formed between two neurons-is critical to mediating information flow in the brain. Synaptic cell adhesion molecules (CAMs) are thought to participate in the initiation of the synapse formation process. However, in vivo functional analysis demonstrates that most well known synaptic CAMs regulate synaptic maturation and plasticity rather than synapse formation, suggesting that either CAMs work synergistically in the process of forming synapses or more CAMs remain to be found. By screening for unknown CAMs using a co-culture system, we revealed that protein tyrosine phosphatase receptor type O (PTPRO) is a potent CAM that induces the formation of artificial synapse clusters in co-cultures of human embryonic kidney 293 cells and hippocampal neurons cultured from newborn mice regardless of gender. PTPRO was enriched in the mouse brain and localized to postsynaptic sites at excitatory synapses. The overexpression of PTPRO in cultured hippocampal neurons increased the number of synapses and the frequency of miniature EPSCs (mEPSCs). The knock-down (KD) of PTPRO expression in cultured neurons by short hairpin RNA (shRNA) reduced the number of synapses and the frequencies of the mEPSCs. The effects of shRNA KD were rescued by expressing either full-length PTPRO or a truncated PTPRO lacking the cytoplasmic domain. Consistent with these results, the N-terminal extracellular domain of PTPRO was required for its synaptogenic activity in the co-culture assay. Our data show that PTPRO is a synaptic CAM that serves as a potent initiator of the formation of excitatory synapses.SIGNIFICANCE STATEMENT The formation of synapses is critical for the brain to execute its function and synaptic cell adhesion molecules (CAMs) play essential roles in initiating the formation of synapses. By screening for unknown CAMs using a co-culture system, we revealed that protein tyrosine phosphatase receptor type O (PTPRO) is a potent CAM that induces the formation of artificial synapse clusters. Using loss-of-function and gain-of-function approaches, we show that PTPRO promotes the formation of excitatory synapses. The N-terminal extracellular domain of PTPRO was required for its synaptogenic activity in cultured hippocampal neurons and the co-culture assay. Together, our data show that PTPRO is a synaptic CAM that serves as a potent initiator of synapse formation." @default.
• W2751976941 created "2017-09-15" @default.
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• W2751976941 date "2017-09-04" @default.
• W2751976941 modified "2023-09-30" @default.
• W2751976941 title "Identification of Protein Tyrosine Phosphatase Receptor Type O (PTPRO) as a Synaptic Adhesion Molecule that Promotes Synapse Formation" @default.
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• W2751976941 cites W1546762987 @default.
• W2751976941 cites W1966896263 @default.
• W2751976941 cites W1973477546 @default.
• W2751976941 cites W1977520891 @default.
• W2751976941 cites W1983541383 @default.
• W2751976941 cites W1983610576 @default.
• W2751976941 cites W1984988598 @default.
• W2751976941 cites W1985702347 @default.
• W2751976941 cites W1988011192 @default.
• W2751976941 cites W1988193376 @default.
• W2751976941 cites W1991366100 @default.
• W2751976941 cites W1992603916 @default.
• W2751976941 cites W1992676399 @default.
• W2751976941 cites W1997791336 @default.
• W2751976941 cites W1999966549 @default.
• W2751976941 cites W2005470116 @default.
• W2751976941 cites W2009321212 @default.
• W2751976941 cites W2015262258 @default.
• W2751976941 cites W2016812630 @default.
• W2751976941 cites W2017577062 @default.
• W2751976941 cites W2019173591 @default.
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• W2751976941 cites W2028058524 @default.
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• W2751976941 cites W2044915994 @default.
• W2751976941 cites W2051434331 @default.
• W2751976941 cites W2055720472 @default.
• W2751976941 cites W2057482484 @default.
• W2751976941 cites W2058544036 @default.
• W2751976941 cites W2058584441 @default.
• W2751976941 cites W2061772901 @default.
• W2751976941 cites W2066188896 @default.
• W2751976941 cites W2068742937 @default.
• W2751976941 cites W2070732190 @default.
• W2751976941 cites W2073156389 @default.
• W2751976941 cites W2086096310 @default.
• W2751976941 cites W2090923280 @default.
• W2751976941 cites W2092932076 @default.
• W2751976941 cites W2096616785 @default.
• W2751976941 cites W2096985729 @default.
• W2751976941 cites W2097394499 @default.
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• W2751976941 cites W2137096994 @default.
• W2751976941 cites W2143423239 @default.
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• W2751976941 doi "https://doi.org/10.1523/jneurosci.0729-17.2017" @default.
• W2751976941 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6596594" @default.
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• W2751976941 hasPublicationYear "2017" @default.
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