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- W2752477674 abstract "We have synthesized charge-neutral, stable, non-cytotoxic, bright, near-infrared (NIR) SnS quantum dots (QDs) by first making Cysteamine-capped SnS QDs in glycerol under acidic conditions followed by lengthening the capping molecule with peptide bonds by reacting the capping molecules with glycine and subsequent heat treatment at 200°C for 4 hours. The obtained stable SnS QDs exhibited a band gap of 1.5 eV and a strong, narrow NIR emission peak at 830 nm with a quantum yield of 4.6%. The suspension could be stable for more than 1 month without aggregation or emission decay. The positively charged SnS QDs were further neutralized by 3-mercaptoproprionic acid (MPA) through electrostatic attraction. The MPA neutralized SnS QDs were shown to be non-cytotoxic at concentrations 6 times the typical QDs concentration for immunostaining. Low-noise, optimal NIR immunofluorescent imaging of vascular endothelial growth factor (VEGF) on 3T3 cells and Tn antigen on HT29 cells was achieved by using streptavidin (SA)-linked MPA-neutralized SnS QDs with a SA:QD molar ratio of 22:1 to bind to biotinylated secondary antibody bound on the primary antibody that was bound on the targeted antigen on the cell membrane with a high signal-to noise ratio (SNR) of 35." @default.
- W2752477674 created "2017-09-15" @default.
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- W2752477674 date "2017-08-22" @default.
- W2752477674 modified "2023-10-13" @default.
- W2752477674 title "Charge‐Neutral, Stable, Non‐Cytotoxic, Near‐Infrared SnS Aqueous Quantum Dots for High Signal‐to‐Noise‐Ratio Biomedical Imaging" @default.
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- W2752477674 doi "https://doi.org/10.1002/slct.201700855" @default.
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