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• W2752589752 abstract "Biomass pretreatment-derived degradation compounds, such as furfural and 5-hydroxymethyl-furfural (HMF), inhibit the growth of fermentation microorganisms that utilize biomass to produce fuels and chemicals. Here we report that recombinant manganese peroxidase (rMnP) produced from the yeast Pichia pastoris can degrade furfural and HMF making them less toxic to microorganisms. Treatment with rMnP or manganese(III) acetate reduced furfural and HMF concentrations in a dose-dependent manner. Furfural disappearance was accompanied by malonate disappearance and accumulation of four distinct degradation products. Furfural was more readily degraded by rMnP and manganese(III) acetate than HMF. Growth assays using Saccharomyces cerevisiae indicated that rMnP treatment reduced the toxicity of furfural and HMF. This work provides an avenue to use rMnP to increase the growth of fermentation microorganisms that are inhibited by toxic compounds derived from pretreatment of biomass." @default.
• W2752589752 created "2017-09-15" @default.
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• W2752589752 date "2018-01-01" @default.
• W2752589752 modified "2023-10-14" @default.
• W2752589752 title "Furfural and 5-hydroxymethyl-furfural degradation using recombinant manganese peroxidase" @default.
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• W2752589752 doi "https://doi.org/10.1016/j.enzmictec.2017.08.009" @default.
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