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- W2752750489 abstract "Supramolecular nanoparticle hybrids for biosensing of analytes have been a major focus due to their tunable optical and surface properties. Quantum dots-Gold nanoparticle (QDs-GNP) based FRET probes involving turn on/off principles have gained immense interest due to their specificity and sensitivity. Recent focus is on applying these supramolecular hybrids for enzyme operated biosensors that can specifically turn-on fluorescence induced by co-factor or product formed from enzymatic reaction. The present study focuses on locking and unlocking the interaction between QD-GNP pair leading to differential fluorescent properties. Cationic GNPs efficiently quenched the anionic QD fluorescence by forming nanoparticle hybrid. Quenching interaction between QD-GNP pair was unlocked by NADH leading to QD fluorescence turn-on. This phenomenon was applied for the successful detection of formaldehyde using NAD+ dependent formaldehyde dehydrogenase. The proposed nano-sniffer could successfully detect formaldehyde from 0.001 to 100000ng/mL (R2 = 0.9339) by the turn off-turn on principle. It could also detect formaldehyde in fruit juice and wine samples indicating its stability and sensitivity in real samples. The proposed nanoprobe can have wide applications in developing enzyme biosensors in future." @default.
- W2752750489 created "2017-09-15" @default.
- W2752750489 creator A5072424387 @default.
- W2752750489 creator A5073525081 @default.
- W2752750489 date "2018-02-01" @default.
- W2752750489 modified "2023-10-16" @default.
- W2752750489 title "Supramolecular nano-sniffers for ultrasensitive detection of formaldehyde" @default.
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- W2752750489 doi "https://doi.org/10.1016/j.bios.2017.09.010" @default.
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