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- W2752803625 abstract "Abstract MicroRNAs (miRNAs) are small noncoding endogenous RNAs, typically 21–23 nucleotides long, that regulate gene expression, usually post-transcriptionally, by binding to the 3′-UTR of target mRNA, thus blocking translation. The expression of several miRNAs is significantly altered during cardiac hypertrophy, myocardial ischemia, fibrosis, heart failure, and other cardiac myopathies. Recent studies have implicated miRNA-9 (miR-9) in myocardial hypertrophy. However, a detailed mechanism remains obscure. In this study, we have addressed the roles of miR-9 in muscle development and function using a genetically tractable model system, the indirect flight muscles (IFMs) of Drosophila melanogaster. Bioinformatics analysis identified 135 potential miR-9a targets, of which 27 genes were associated with Drosophila muscle development. Troponin-T (TnT) was identified as major structural gene target of miR-9a. We show that flies overexpressing miR-9a in the IFMs have abnormal wing position and are flightless. These flies also exhibit a loss of muscle integrity and sarcomeric organization causing an abnormal muscle condition known as “hypercontraction.” Additionally, miR-9a overexpression resulted in the reduction of TnT protein levels while transcript levels were unaffected. Furthermore, muscle abnormalities associated with miR-9a overexpression were completely rescued by overexpression of TnT transgenes which lacked the miR-9a binding site. These findings indicate that miR-9a interacts with the 3′-UTR of the TnT mRNA and downregulates the TnT protein levels by translational repression. The reduction in TnT levels leads to a cooperative downregulation of other thin filament structural proteins. Our findings have implications for understanding the cellular pathophysiology of cardiomyopathies associated with miR-9 overexpression." @default.
- W2752803625 created "2017-09-15" @default.
- W2752803625 creator A5013371189 @default.
- W2752803625 creator A5040789180 @default.
- W2752803625 creator A5062891855 @default.
- W2752803625 creator A5082092300 @default.
- W2752803625 date "2017-10-01" @default.
- W2752803625 modified "2023-10-16" @default.
- W2752803625 title "Overexpression of miRNA-9 Generates Muscle Hypercontraction Through Translational Repression of Troponin-T in<i>Drosophila melanogaster</i>Indirect Flight Muscles" @default.
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- W2752803625 doi "https://doi.org/10.1534/g3.117.300232" @default.
- W2752803625 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5633399" @default.
- W2752803625 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28866639" @default.
- W2752803625 hasPublicationYear "2017" @default.
- W2752803625 type Work @default.