Matches in SemOpenAlex for { <https://semopenalex.org/work/W2752912644> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2752912644 endingPage "79" @default.
- W2752912644 startingPage "79" @default.
- W2752912644 abstract "<ns4:p><ns4:bold>Background:</ns4:bold> Immunofluorescence (IF) is one of the most important techniques where fluorochromes conjugated to antibodies are used to detect specific proteins or antigens. In tissue sections, autofluorescence (AF) can lead to poor quality images that impair assessment. The placenta is a pivotal extra-embryonic organ in embryo development, where trophoblasts make up a large proportion of the cells. Teratoma formation is one of the critical assays for pluripotent stem cells.</ns4:p><ns4:p> <ns4:bold>Methods:</ns4:bold> We tested whether ultraviolet (UV), ammonia (NH3), copper (II) sulfate (CuSO4), Trypan Blue (TB), Sudan Black B (SB), TrueBlack™ Lipofusin Autofluorescence Quencher (TLAQ) and combinations of these treatments could reduce AF in paraffin and frozen sections of placenta and teratoma in FITC, Texas Red and Cy5.5 channels.</ns4:p><ns4:p> <ns4:bold>Results: </ns4:bold>We found that UV, NH3, TB and CuSO4 quenched AF to some extent in different tissue and filters, but increased AF in Texas Red or Cy5.5 channels in some cases. SB and TLQA exhibited the most consistent effects on decreasing AF, though TLQA reduced the overall IF signal in placenta sections. Not all combined treatments further reduced AF in both placenta and teratoma sections.</ns4:p><ns4:p> <ns4:bold>Conclusions:</ns4:bold> SB and TLAQ can effectively quench AF in placenta and teratoma IF.</ns4:p>" @default.
- W2752912644 created "2017-09-15" @default.
- W2752912644 creator A5002055593 @default.
- W2752912644 creator A5014067611 @default.
- W2752912644 creator A5015985377 @default.
- W2752912644 creator A5037778417 @default.
- W2752912644 creator A5076250197 @default.
- W2752912644 creator A5089015655 @default.
- W2752912644 creator A5091468568 @default.
- W2752912644 date "2017-09-04" @default.
- W2752912644 modified "2023-10-15" @default.
- W2752912644 title "Quenching autofluorescence in tissue immunofluorescence" @default.
- W2752912644 cites W1486131515 @default.
- W2752912644 cites W1486376079 @default.
- W2752912644 cites W1521853259 @default.
- W2752912644 cites W1965067847 @default.
- W2752912644 cites W1970508192 @default.
- W2752912644 cites W1971453554 @default.
- W2752912644 cites W1990427469 @default.
- W2752912644 cites W2003179114 @default.
- W2752912644 cites W2007015982 @default.
- W2752912644 cites W2007295158 @default.
- W2752912644 cites W2009727012 @default.
- W2752912644 cites W2019058903 @default.
- W2752912644 cites W2058068800 @default.
- W2752912644 cites W2079985607 @default.
- W2752912644 cites W2089378200 @default.
- W2752912644 cites W2092772483 @default.
- W2752912644 cites W2094697647 @default.
- W2752912644 cites W2101822059 @default.
- W2752912644 cites W2105674793 @default.
- W2752912644 cites W2129006023 @default.
- W2752912644 cites W2137405382 @default.
- W2752912644 cites W2138417516 @default.
- W2752912644 cites W2291619526 @default.
- W2752912644 cites W4376453255 @default.
- W2752912644 doi "https://doi.org/10.12688/wellcomeopenres.12251.1" @default.
- W2752912644 hasPublicationYear "2017" @default.
- W2752912644 type Work @default.
- W2752912644 sameAs 2752912644 @default.
- W2752912644 citedByCount "12" @default.
- W2752912644 countsByYear W27529126442017 @default.
- W2752912644 countsByYear W27529126442018 @default.
- W2752912644 countsByYear W27529126442020 @default.
- W2752912644 countsByYear W27529126442021 @default.
- W2752912644 countsByYear W27529126442022 @default.
- W2752912644 countsByYear W27529126442023 @default.
- W2752912644 crossrefType "journal-article" @default.
- W2752912644 hasAuthorship W2752912644A5002055593 @default.
- W2752912644 hasAuthorship W2752912644A5014067611 @default.
- W2752912644 hasAuthorship W2752912644A5015985377 @default.
- W2752912644 hasAuthorship W2752912644A5037778417 @default.
- W2752912644 hasAuthorship W2752912644A5076250197 @default.
- W2752912644 hasAuthorship W2752912644A5089015655 @default.
- W2752912644 hasAuthorship W2752912644A5091468568 @default.
- W2752912644 hasBestOaLocation W27529126441 @default.
- W2752912644 hasConcept C121332964 @default.
- W2752912644 hasConcept C172680121 @default.
- W2752912644 hasConcept C179687394 @default.
- W2752912644 hasConcept C2776953305 @default.
- W2752912644 hasConcept C2779234561 @default.
- W2752912644 hasConcept C54355233 @default.
- W2752912644 hasConcept C62520636 @default.
- W2752912644 hasConcept C86803240 @default.
- W2752912644 hasConcept C91881484 @default.
- W2752912644 hasConceptScore W2752912644C121332964 @default.
- W2752912644 hasConceptScore W2752912644C172680121 @default.
- W2752912644 hasConceptScore W2752912644C179687394 @default.
- W2752912644 hasConceptScore W2752912644C2776953305 @default.
- W2752912644 hasConceptScore W2752912644C2779234561 @default.
- W2752912644 hasConceptScore W2752912644C54355233 @default.
- W2752912644 hasConceptScore W2752912644C62520636 @default.
- W2752912644 hasConceptScore W2752912644C86803240 @default.
- W2752912644 hasConceptScore W2752912644C91881484 @default.
- W2752912644 hasFunder F4320307874 @default.
- W2752912644 hasLocation W27529126441 @default.
- W2752912644 hasOpenAccess W2752912644 @default.
- W2752912644 hasPrimaryLocation W27529126441 @default.
- W2752912644 hasRelatedWork W2016081239 @default.
- W2752912644 hasRelatedWork W2027376101 @default.
- W2752912644 hasRelatedWork W2048484557 @default.
- W2752912644 hasRelatedWork W2069784239 @default.
- W2752912644 hasRelatedWork W2082860237 @default.
- W2752912644 hasRelatedWork W2098293184 @default.
- W2752912644 hasRelatedWork W2140197046 @default.
- W2752912644 hasRelatedWork W2369270460 @default.
- W2752912644 hasRelatedWork W2400218427 @default.
- W2752912644 hasRelatedWork W3198649021 @default.
- W2752912644 hasVolume "2" @default.
- W2752912644 isParatext "false" @default.
- W2752912644 isRetracted "false" @default.
- W2752912644 magId "2752912644" @default.
- W2752912644 workType "article" @default.