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• W2753199118 abstract "Abstract The detection of bacteria cells and their viability in food, water and clinical samples is critical to bioscience research and biomedical practice. In this work, we present a microfluidic device encapsulating a coplanar waveguide for differentiation of live and heat-killed Escherichia coli cells suspended in culture media using microwave signals over the frequency range of 0.5–20 GHz. From small populations of ∼15 E. coli cells, both the transmitted (|S21|) and reflected (|S11|) microwave signals show a difference between live and dead populations, with the difference especially significant for |S21| below 10 GHz. Analysis based on an equivalent circuit suggests that the difference is due to a reduction of the cytoplasm conductance and permittivity upon cell death. The electrical measurement is confirmed by off-chip biochemical analysis: the conductivity of cell lysate from heat-killed E. coli is 8.22% lower than that from viable cells. Furthermore, protein diffusivity increases in the cytoplasm of dead cells, suggesting the loss of cytoplasmic compactness. These changes are results of intact cell membrane of live cells acting as a semipermeable barrier, within which ion concentration and macromolecule species are tightly regulated. On the other hand, the cell membrane of dead cells is compromised, allowing ions and molecules to leak out of the cytoplasm. The loss of cytoplasmic content as well as membrane integrity is measurable by microwave impedance sensors. Since our approach allows detection of bacterial viability in the native growth environment, it is a promising strategy for rapid point-of-care diagnostics of microorganisms as well as sensing biological agents in bioterrorism and food safety threats." @default.
• W2753199118 created "2017-09-15" @default.
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• W2753199118 date "2018-02-01" @default.
• W2753199118 modified "2023-10-17" @default.
• W2753199118 title "Differentiation of live and heat-killed E. coli by microwave impedance spectroscopy" @default.
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• W2753199118 doi "https://doi.org/10.1016/j.snb.2017.08.179" @default.
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