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- W2753362147 abstract "Background and objective Harpullia pendula Planch leaves belong to the Sapindaceae family. The study aimed to investigate the phenolic constituents and evaluate the antioxidant and α-amylase inhibitory activities of the plant’s extracts and its major compounds.Materials and methods The compounds were isolated through chromatographic techniques from the defatted ethanolic extract (DAEE). Their structures were determined by ultraviolet, mass spectrometer, and nuclear magnetic resonance spectroscopy.Results The flavonoids kaempferol 3-O-(6″galloyl)- apiofuranosyl (1‴→2″)-β-galactopyranoside, kaempferol-3-O-β-glucopyranosyl(1‴→6″)-β-glucopyranoside, kaempferol 3-O-(6″galloyl)-apiofuranosyl (1‴→2″)-β-galactopyranoside, rutin, vitexin, isovitexin, orientin, quercetin, kaempferol; the tannins ellagic acid, gallic acid, methyl gallate, 2,6-di-O-galloyl(α/β)glucoside, 2,3-di-O-galloyl(α/β)glucoside, and tetragalloyl glucoside in addition to two benzene acetic acid derivatives, harpulliaside A and cavaol B, were isolated from the total bioactive ethanolic extract (TEE). The TEE and the DAEE of H. pendula have a total phenolic content of 255.5±7.18 and 222.9±6.43 mg gallic acid equivalents/g extract, respectively, and a total flavonoid content of 111.6±3.2 and 102.6±2.6 mg quercetin equivalents/g extract, respectively. With respect to the in vitro study, DAEE, TEE, and methyl gallate showed an interesting inhibitory activity on 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) [half maximal inhibitory concentration (IC50): 13.3±0.4, 17.7±0.7, and 19.4±0.08 µg/ml, respectively], nitric oxide (IC50: 12.8±2.54, 18.3±1.6, and 29.8±1.00 µg/ml, respectively), and α-amylase (IC50: 6.1±0.554, 14.4±0.681, and17.5±0.003 µg/ml, respectively).Conclusion H. pendula extracts are rich in phenolic compounds; the aforementioned results suggest that DAEE, TEE, and methyl gallate may be potentially useful in hegemony of obesity and diabetes." @default.
- W2753362147 created "2017-09-15" @default.
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- W2753362147 date "2017-01-01" @default.
- W2753362147 modified "2023-09-27" @default.
- W2753362147 title "Harpullia pendula Planch leaves: phenolics, in vitro antioxidant and α-amylase inhibitory activity" @default.
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- W2753362147 doi "https://doi.org/10.4103/epj.epj_10_17" @default.
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