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• W2753485238 abstract "In this in vitro gradient perfusion study, we determined whether there is a functional relationship between oestradiol and the arginine vasotocin/isotocin (AVT/IT) system in the female round goby (Neogobius melanostomus). Brain explants were perfused in medium supplemented with 17β-oestradiol (E2) at doses mimicking the plasma levels of this hormone in nature during the spawning-capable phase and regressing phase. We aimed to establish which pathway, genomic or non-genomic, is involved in this mechanism in different reproductive phases. For this purpose, brain explants were perfused in medium supplemented with Fulvestrant (ICI 182.780) or Actinomycin D (Act D) separately or in combination with E2. The contents of AVT and IT in the perfusion media were determined using high-performance liquid chromatography (HPLC) with fluorescence and UV detection. During the spawning-capable phase, the effect of E2 on AVT release is mediated through oestrogen receptors (ERs) via both genomic and non-genomic pathways, while IT release is mediated through ERs via a genomic pathway only. In the regressing phase, release of both nonapeptides is mediated through ERs via a genomic pathway. These are the first studies presenting a feasible mechanism of oestradiol action on the AVT/IT system in female fish during different phases of the reproductive cycle." @default.
• W2753485238 created "2017-09-15" @default.
• W2753485238 creator A5052109628 @default.
• W2753485238 creator A5081280438 @default.
• W2753485238 creator A5083883222 @default.
• W2753485238 creator A5084523030 @default.
• W2753485238 date "2017-01-01" @default.
• W2753485238 modified "2023-09-30" @default.
• W2753485238 title "How does oestradiol influence the AVT/IT system in female round gobies during different reproductive phases?" @default.
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• W2753485238 doi "https://doi.org/10.1242/bio.024844" @default.
• W2753485238 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5665460" @default.
• W2753485238 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28860130" @default.

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