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- W2753647037 abstract "The aim of this study was to determine effect of vanadate on glucose production in hepatocytes cultured in vitro. Hepatocytes were isolated by a collagenase perfusion technique and cultured for 24 h in M 199 serum-free medium. The glucose production was measured by incubating the cultures in glucose-free Hanks-Hepes medium with addition 10 mmol/l pyruvate. The glucose released into the medium was determined enzymatically with glucose oxidase. Glycogenolysis was determined as net decrease in glycogen content during the incubation period. Vanadate alone in a concentration of 1 mmol/l caused a significant (p<0.01) 57% increase of glucose production during first hour, but after that period glucose production complete ceased and hepatocytes started to consume glucose, showed as a steadily and slow decrease of glucose in medium. Although insulin alone did not change glucose production, vanadate in presence of insulin even stronger (80%) increase glucose production during first hour, but after that very strongly (60%) decreased glucose in medium during second hour. Results obtained showed that vanadate decreased glucose production inhibiting gluconeogenesis" @default.
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- W2753647037 date "2004-01-01" @default.
- W2753647037 modified "2023-09-24" @default.
- W2753647037 title "Effects of vanadate on glucose production in rat hepatocytes cultured in vitro" @default.
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