FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2753709543> ?p ?o ?g. }

• W2753709543 endingPage "1986" @default.
• W2753709543 startingPage "1975" @default.
• W2753709543 abstract "We have previously reported that myeloid differentiation primary response gene 88 (MyD88) is downregulated during all-trans retinoic acid (RA)-induced differentiation of pluripotent NTera2 human embryonal carcinoma cells (hECCs), whereas its maintained expression is associated with RA differentiation resistance in nullipotent 2102Ep hECCs. MyD88 is the main adapter for toll-like receptor (TLR) signalling, where it determines the secretion of chemokines and cytokines in response to pathogens. In this study, we report that loss of MyD88 is essential for RA-facilitated differentiation of hECCs. Functional analysis using a specific MyD88 peptide inhibitor (PepInh) demonstrated that high MyD88 expression in the self-renewal state inhibits the expression of a specific set of HOX genes. In NTera2 cells, MyD88 is downregulated during RA-induced differentiation, a mechanism that could be broadly replicated by MyD88 PepInh treatment of 2102Ep cells. Notably, MyD88 inhibition transitioned 2102Ep cells into a stable, self-renewing state that appears to be primed for differentiation upon addition of RA. At a molecular level, MyD88 inhibition combined with RA treatment upregulated HOX, RA signalling and TLR signalling genes. These events permit differentiation through a standard downregulation of Oct4-Sox2-Nanog mechanism. In line with its role in regulating secretion of specific proteins, conditioned media experiments demonstrated that differentiated (MyD88 low) NTera2 cell media was sufficient to differentiate NTera2 cells. Protein array analysis indicated that this was owing to secretion of factors known to regulate angiogenesis, neurogenesis and all three branches of TGF-β Superfamily signalling. Collectively, these data offer new insights into RA controlled differentiation of pluripotent cells, with notable parallels to the ground state model of embryonic stem cell self-renewal. These data may provide insights to facilitate improved differentiation protocols for regenerative medicine and differentiation-therapies in cancer treatment." @default.
• W2753709543 created "2017-09-15" @default.
• W2753709543 creator A5001697382 @default.
• W2753709543 creator A5009977791 @default.
• W2753709543 creator A5010388111 @default.
• W2753709543 creator A5012732492 @default.
• W2753709543 creator A5014877332 @default.
• W2753709543 creator A5025174038 @default.
• W2753709543 creator A5026179000 @default.
• W2753709543 creator A5028387712 @default.
• W2753709543 creator A5041987677 @default.
• W2753709543 creator A5043078189 @default.
• W2753709543 creator A5043621658 @default.
• W2753709543 creator A5045651335 @default.
• W2753709543 creator A5052089056 @default.
• W2753709543 creator A5054886488 @default.
• W2753709543 creator A5061739582 @default.
• W2753709543 creator A5062491786 @default.
• W2753709543 creator A5064191932 @default.
• W2753709543 creator A5065134050 @default.
• W2753709543 creator A5065279844 @default.
• W2753709543 creator A5065688806 @default.
• W2753709543 creator A5066196777 @default.
• W2753709543 creator A5073330922 @default.
• W2753709543 creator A5076103229 @default.
• W2753709543 creator A5076461186 @default.
• W2753709543 creator A5078256751 @default.
• W2753709543 date "2017-09-08" @default.
• W2753709543 modified "2023-10-16" @default.
• W2753709543 title "MyD88 is an essential component of retinoic acid-induced differentiation in human pluripotent embryonal carcinoma cells" @default.
• W2753709543 cites W1927684145 @default.
• W2753709543 cites W1965647040 @default.
• W2753709543 cites W1971888188 @default.
• W2753709543 cites W1972524782 @default.
• W2753709543 cites W1972880938 @default.
• W2753709543 cites W1975805528 @default.
• W2753709543 cites W1994322768 @default.
• W2753709543 cites W1995339453 @default.
• W2753709543 cites W1995586746 @default.
• W2753709543 cites W1996120614 @default.
• W2753709543 cites W2001525581 @default.
• W2753709543 cites W2008289938 @default.
• W2753709543 cites W2024042582 @default.
• W2753709543 cites W2030678455 @default.
• W2753709543 cites W2033960534 @default.
• W2753709543 cites W2045899482 @default.
• W2753709543 cites W2055898299 @default.
• W2753709543 cites W2062835892 @default.
• W2753709543 cites W2064906046 @default.
• W2753709543 cites W2069158810 @default.
• W2753709543 cites W2070597700 @default.
• W2753709543 cites W2076355203 @default.
• W2753709543 cites W2079625772 @default.
• W2753709543 cites W2086467940 @default.
• W2753709543 cites W2086943348 @default.
• W2753709543 cites W2087143957 @default.
• W2753709543 cites W2095945017 @default.
• W2753709543 cites W2106945543 @default.
• W2753709543 cites W2113680845 @default.
• W2753709543 cites W2115903163 @default.
• W2753709543 cites W2118983602 @default.
• W2753709543 cites W2119656037 @default.
• W2753709543 cites W2122621344 @default.
• W2753709543 cites W2123922822 @default.
• W2753709543 cites W2126100547 @default.
• W2753709543 cites W2133616332 @default.
• W2753709543 cites W2144120657 @default.
• W2753709543 cites W2152802057 @default.
• W2753709543 cites W2158217645 @default.
• W2753709543 cites W2165996867 @default.
• W2753709543 cites W2168191559 @default.
• W2753709543 cites W2256214571 @default.
• W2753709543 cites W2288812985 @default.
• W2753709543 cites W2331693254 @default.
• W2753709543 cites W2407345634 @default.
• W2753709543 cites W2418627005 @default.
• W2753709543 cites W4236316592 @default.
• W2753709543 cites W4251635267 @default.
• W2753709543 cites W4290209684 @default.
• W2753709543 doi "https://doi.org/10.1038/cdd.2017.124" @default.
• W2753709543 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5635222" @default.
• W2753709543 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28885616" @default.
• W2753709543 hasPublicationYear "2017" @default.
• W2753709543 type Work @default.
• W2753709543 sameAs 2753709543 @default.
• W2753709543 citedByCount "5" @default.
• W2753709543 countsByYear W27537095432020 @default.
• W2753709543 countsByYear W27537095432023 @default.
• W2753709543 crossrefType "journal-article" @default.
• W2753709543 hasAuthorship W2753709543A5001697382 @default.
• W2753709543 hasAuthorship W2753709543A5009977791 @default.
• W2753709543 hasAuthorship W2753709543A5010388111 @default.
• W2753709543 hasAuthorship W2753709543A5012732492 @default.
• W2753709543 hasAuthorship W2753709543A5014877332 @default.
• W2753709543 hasAuthorship W2753709543A5025174038 @default.
• W2753709543 hasAuthorship W2753709543A5026179000 @default.
• W2753709543 hasAuthorship W2753709543A5028387712 @default.
• W2753709543 hasAuthorship W2753709543A5041987677 @default.

• next