Matches in SemOpenAlex for { <https://semopenalex.org/work/W2754222317> ?p ?o ?g. }
- W2754222317 endingPage "90" @default.
- W2754222317 startingPage "83" @default.
- W2754222317 abstract "Edwardsiella ictaluri is a Gram-negative facultative intracellular pathogen causing enteric septicemia of channel catfish (ESC). Our recent work indicated that tricarboxylic acid cycle and one-carbon metabolism are critical pathways for E. ictaluri virulence. Although single and double gene deletions in these pathways resulted in safe and efficacious vaccines for use in catfish fingerlings, vaccine trials in catfish fry showed safety concerns. Therefore, we aimed to improve the safety of these mutants by constructing two triple mutant combinations. ESC-NDKL1 (ΔgcvPΔsdhCΔfrdA) was constructed by introducing an in-frame deletion of frdA in a gcvP-sdh mutant. ESC-NDKL2 (ΔgcvPΔsdhCΔmdh) was constructed in a similar manner. ESC-NDKL1 strain was a better vaccine candidate compared to ESC-NDKL2, providing better safety and efficacy in catfish fry and catfish fingerlings. Field trials in earthen ponds under three vaccination conditions showed that survival was significantly higher in catfish vaccinated with ESC-NDKL1 by immersion at the fry stage, oral vaccination in ponds, and fry immersion-pond oral combination (86.74%, 81.67%, and 95.22%, respectively) compared to sham-vaccinated (42.75%), and Aquavac-ESC fry immersion vaccinated (61.51%) catfish. Our findings indicate that ESC-NDKL1 is a good candidate for further development as a vaccine for ESC." @default.
- W2754222317 created "2017-09-25" @default.
- W2754222317 creator A5020539146 @default.
- W2754222317 creator A5030994049 @default.
- W2754222317 creator A5053283242 @default.
- W2754222317 creator A5075574028 @default.
- W2754222317 date "2017-10-01" @default.
- W2754222317 modified "2023-10-18" @default.
- W2754222317 title "Improving safety of a live attenuated Edwardsiella ictaluri vaccine against enteric septicemia of catfish and evaluation of efficacy" @default.
- W2754222317 cites W1556360002 @default.
- W2754222317 cites W1593691014 @default.
- W2754222317 cites W1787690277 @default.
- W2754222317 cites W1824614244 @default.
- W2754222317 cites W1869227722 @default.
- W2754222317 cites W1914174905 @default.
- W2754222317 cites W1966327123 @default.
- W2754222317 cites W1979918990 @default.
- W2754222317 cites W1987522521 @default.
- W2754222317 cites W1995877713 @default.
- W2754222317 cites W2003623016 @default.
- W2754222317 cites W2006023877 @default.
- W2754222317 cites W2007052904 @default.
- W2754222317 cites W2010311067 @default.
- W2754222317 cites W2024121963 @default.
- W2754222317 cites W2041918033 @default.
- W2754222317 cites W2042490028 @default.
- W2754222317 cites W2045707865 @default.
- W2754222317 cites W2045735260 @default.
- W2754222317 cites W2046524869 @default.
- W2754222317 cites W2064006139 @default.
- W2754222317 cites W2064496380 @default.
- W2754222317 cites W2066101287 @default.
- W2754222317 cites W2068476601 @default.
- W2754222317 cites W2084880502 @default.
- W2754222317 cites W2089054937 @default.
- W2754222317 cites W2090367376 @default.
- W2754222317 cites W2099186794 @default.
- W2754222317 cites W2112296388 @default.
- W2754222317 cites W2113204163 @default.
- W2754222317 cites W2116606702 @default.
- W2754222317 cites W2122445172 @default.
- W2754222317 cites W2123117472 @default.
- W2754222317 cites W2138333173 @default.
- W2754222317 cites W2168997585 @default.
- W2754222317 cites W2292048517 @default.
- W2754222317 cites W2068135038 @default.
- W2754222317 doi "https://doi.org/10.1016/j.vetmic.2017.09.004" @default.
- W2754222317 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29103702" @default.
- W2754222317 hasPublicationYear "2017" @default.
- W2754222317 type Work @default.
- W2754222317 sameAs 2754222317 @default.
- W2754222317 citedByCount "14" @default.
- W2754222317 countsByYear W27542223172018 @default.
- W2754222317 countsByYear W27542223172019 @default.
- W2754222317 countsByYear W27542223172020 @default.
- W2754222317 countsByYear W27542223172021 @default.
- W2754222317 countsByYear W27542223172022 @default.
- W2754222317 crossrefType "journal-article" @default.
- W2754222317 hasAuthorship W2754222317A5020539146 @default.
- W2754222317 hasAuthorship W2754222317A5030994049 @default.
- W2754222317 hasAuthorship W2754222317A5053283242 @default.
- W2754222317 hasAuthorship W2754222317A5075574028 @default.
- W2754222317 hasConcept C104317684 @default.
- W2754222317 hasConcept C159047783 @default.
- W2754222317 hasConcept C22070199 @default.
- W2754222317 hasConcept C2778189562 @default.
- W2754222317 hasConcept C2778949639 @default.
- W2754222317 hasConcept C2909208804 @default.
- W2754222317 hasConcept C2911177997 @default.
- W2754222317 hasConcept C505870484 @default.
- W2754222317 hasConcept C54355233 @default.
- W2754222317 hasConcept C54602769 @default.
- W2754222317 hasConcept C60987743 @default.
- W2754222317 hasConcept C86803240 @default.
- W2754222317 hasConcept C89423630 @default.
- W2754222317 hasConceptScore W2754222317C104317684 @default.
- W2754222317 hasConceptScore W2754222317C159047783 @default.
- W2754222317 hasConceptScore W2754222317C22070199 @default.
- W2754222317 hasConceptScore W2754222317C2778189562 @default.
- W2754222317 hasConceptScore W2754222317C2778949639 @default.
- W2754222317 hasConceptScore W2754222317C2909208804 @default.
- W2754222317 hasConceptScore W2754222317C2911177997 @default.
- W2754222317 hasConceptScore W2754222317C505870484 @default.
- W2754222317 hasConceptScore W2754222317C54355233 @default.
- W2754222317 hasConceptScore W2754222317C54602769 @default.
- W2754222317 hasConceptScore W2754222317C60987743 @default.
- W2754222317 hasConceptScore W2754222317C86803240 @default.
- W2754222317 hasConceptScore W2754222317C89423630 @default.
- W2754222317 hasLocation W27542223171 @default.
- W2754222317 hasLocation W27542223172 @default.
- W2754222317 hasOpenAccess W2754222317 @default.
- W2754222317 hasPrimaryLocation W27542223171 @default.
- W2754222317 hasRelatedWork W1764699798 @default.
- W2754222317 hasRelatedWork W1973021631 @default.
- W2754222317 hasRelatedWork W2045735260 @default.
- W2754222317 hasRelatedWork W2111454258 @default.
- W2754222317 hasRelatedWork W2117700646 @default.
- W2754222317 hasRelatedWork W2123117472 @default.