Matches in SemOpenAlex for { <https://semopenalex.org/work/W2754266919> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W2754266919 abstract "Background Gout is the most prevalent inflammatory arthropathy in young men, in which clinical manifestations are triggered by the deposits of monosodium urate (MSU) crystals in and around joints. From a clinical perspective, gouty attacks and tophaceous deposits have been associated to nodal osteoarthritis [1,2]. Objectives To determine the molecular mechanisms associating MSU crystal deposits with chondrocyte activation and changes in articular cartilage. Methods Primary cultures of synoviocytes were activated with MSU crystals for 24 h, the resulting supernatant was used as a supplementary conditioning basal medium (CBM) for 24 h chondrocyte growth. The expression level profile of cytokines (IL-6, IL-8) and nerve growth factor (NGF) in the supernatant was measured. Production of reactive oxygen and nitrogen species, such as O2●, H2O2, and NO●, was also measured intracellularly. Results Upon activation by MSU crystals, synoviocytes increased secretion of IL-6 and IL-8, as well as intracellular production of O2●, H2O2, and NO● in comparison to non-stimulated synoviocytes. Additionally, the chondrocytes incubated with CBM for 24 h increased significantly IL-6, IL-8, and NGF levels, as well as O2●, H2O2, and NO● levels when compared to non-stimulated chondrocytes. Secretion of these soluble mediators was even higher than the one observed in the synoviocytes and chondrocytes directly stimulated with MSU crystals. Conclusions Pro-inflammatory cytokine secretion induced by MSU crystals in synoviocytes triggers chondrocyte activation, intensifying the articular inflammatory state in gouty attacks. Furthermore, the increase in reactive species (particularly NO●, which inhibits proteoglycan synthesis) compromises the homeostasis of the extracellular matrix of the cartilage. Finally, overproduction of NGF and H2O2 by the chondrocyte functions a pain modulator during gout attacks. References Fam AG, et al. Gouty arthritis in nodal osteoarthritis. J Rheumatol 1996; 23:684–9. Roddy E, et al. Gout and nodal osteoarthritis: a case-control study. Rheumatology (Oxford) 2008; 47:732–3. Disclosure of Interest None declared" @default.
- W2754266919 created "2017-09-25" @default.
- W2754266919 creator A5007718516 @default.
- W2754266919 creator A5008000761 @default.
- W2754266919 creator A5018870826 @default.
- W2754266919 creator A5019811024 @default.
- W2754266919 creator A5046993841 @default.
- W2754266919 creator A5052584081 @default.
- W2754266919 creator A5059437983 @default.
- W2754266919 creator A5067207076 @default.
- W2754266919 creator A5069759691 @default.
- W2754266919 creator A5070323063 @default.
- W2754266919 creator A5088689407 @default.
- W2754266919 creator A5089971687 @default.
- W2754266919 creator A5090953579 @default.
- W2754266919 date "2017-06-01" @default.
- W2754266919 modified "2023-09-24" @default.
- W2754266919 title "AB0059 Synovial secretion of pro-inflammatory and pro-oxidant molecules triggered by monosodium urate crystals induces ngf and h2o2 pain mediators in the chondrocyte" @default.
- W2754266919 doi "https://doi.org/10.1136/annrheumdis-2017-eular.2401" @default.
- W2754266919 hasPublicationYear "2017" @default.
- W2754266919 type Work @default.
- W2754266919 sameAs 2754266919 @default.
- W2754266919 citedByCount "0" @default.
- W2754266919 crossrefType "proceedings-article" @default.
- W2754266919 hasAuthorship W2754266919A5007718516 @default.
- W2754266919 hasAuthorship W2754266919A5008000761 @default.
- W2754266919 hasAuthorship W2754266919A5018870826 @default.
- W2754266919 hasAuthorship W2754266919A5019811024 @default.
- W2754266919 hasAuthorship W2754266919A5046993841 @default.
- W2754266919 hasAuthorship W2754266919A5052584081 @default.
- W2754266919 hasAuthorship W2754266919A5059437983 @default.
- W2754266919 hasAuthorship W2754266919A5067207076 @default.
- W2754266919 hasAuthorship W2754266919A5069759691 @default.
- W2754266919 hasAuthorship W2754266919A5070323063 @default.
- W2754266919 hasAuthorship W2754266919A5088689407 @default.
- W2754266919 hasAuthorship W2754266919A5089971687 @default.
- W2754266919 hasAuthorship W2754266919A5090953579 @default.
- W2754266919 hasBestOaLocation W27542669191 @default.
- W2754266919 hasConcept C105702510 @default.
- W2754266919 hasConcept C126322002 @default.
- W2754266919 hasConcept C134018914 @default.
- W2754266919 hasConcept C142724271 @default.
- W2754266919 hasConcept C164027704 @default.
- W2754266919 hasConcept C203014093 @default.
- W2754266919 hasConcept C204787440 @default.
- W2754266919 hasConcept C2776164576 @default.
- W2754266919 hasConcept C2776914184 @default.
- W2754266919 hasConcept C2778690821 @default.
- W2754266919 hasConcept C2780550940 @default.
- W2754266919 hasConcept C2781403057 @default.
- W2754266919 hasConcept C48349386 @default.
- W2754266919 hasConcept C49039625 @default.
- W2754266919 hasConcept C71924100 @default.
- W2754266919 hasConcept C86803240 @default.
- W2754266919 hasConcept C95444343 @default.
- W2754266919 hasConceptScore W2754266919C105702510 @default.
- W2754266919 hasConceptScore W2754266919C126322002 @default.
- W2754266919 hasConceptScore W2754266919C134018914 @default.
- W2754266919 hasConceptScore W2754266919C142724271 @default.
- W2754266919 hasConceptScore W2754266919C164027704 @default.
- W2754266919 hasConceptScore W2754266919C203014093 @default.
- W2754266919 hasConceptScore W2754266919C204787440 @default.
- W2754266919 hasConceptScore W2754266919C2776164576 @default.
- W2754266919 hasConceptScore W2754266919C2776914184 @default.
- W2754266919 hasConceptScore W2754266919C2778690821 @default.
- W2754266919 hasConceptScore W2754266919C2780550940 @default.
- W2754266919 hasConceptScore W2754266919C2781403057 @default.
- W2754266919 hasConceptScore W2754266919C48349386 @default.
- W2754266919 hasConceptScore W2754266919C49039625 @default.
- W2754266919 hasConceptScore W2754266919C71924100 @default.
- W2754266919 hasConceptScore W2754266919C86803240 @default.
- W2754266919 hasConceptScore W2754266919C95444343 @default.
- W2754266919 hasLocation W27542669191 @default.
- W2754266919 hasOpenAccess W2754266919 @default.
- W2754266919 hasPrimaryLocation W27542669191 @default.
- W2754266919 hasRelatedWork W1985069706 @default.
- W2754266919 hasRelatedWork W2027870593 @default.
- W2754266919 hasRelatedWork W2033088300 @default.
- W2754266919 hasRelatedWork W2055114626 @default.
- W2754266919 hasRelatedWork W2088903390 @default.
- W2754266919 hasRelatedWork W2093568382 @default.
- W2754266919 hasRelatedWork W2095103574 @default.
- W2754266919 hasRelatedWork W2115100406 @default.
- W2754266919 hasRelatedWork W2170170036 @default.
- W2754266919 hasRelatedWork W2322250467 @default.
- W2754266919 hasRelatedWork W2412688823 @default.
- W2754266919 hasRelatedWork W2419169211 @default.
- W2754266919 hasRelatedWork W2422921680 @default.
- W2754266919 hasRelatedWork W2511117452 @default.
- W2754266919 hasRelatedWork W2613643891 @default.
- W2754266919 hasRelatedWork W2777181382 @default.
- W2754266919 hasRelatedWork W3082811178 @default.
- W2754266919 hasRelatedWork W3097502238 @default.
- W2754266919 hasRelatedWork W3119729301 @default.
- W2754266919 hasRelatedWork W3151261802 @default.
- W2754266919 isParatext "false" @default.
- W2754266919 isRetracted "false" @default.
- W2754266919 magId "2754266919" @default.
- W2754266919 workType "article" @default.