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• W2754396002 abstract "Second-generation RNA-based fluorescent biosensorsbiosensors have been developed that enable flow cytometryflow cytometry experiments to monitor the population dynamics of c-di-GMP signaling in live bacteria. These experiments are high-throughput, provide information at the single-cell level, and can be performed on cells grown in complex media and/or under anaerobic conditions. Here, we describe flow cytometry methods for three applications: (1) high-throughput screeningscreening for diguanylate cyclase activity, (2) analyzing c-di-GMP levels under anaerobic conditions, and (3) monitoring cell population dynamics of c-di-GMP levels upon environmental changes. These methods showcase RNA-based fluorescent biosensors as versatile tools for studying c-di-GMP signaling in bacteria." @default.
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• W2754396002 date "2017-01-01" @default.
• W2754396002 modified "2023-09-26" @default.
• W2754396002 title "Live Flow Cytometry Analysis of c-di-GMP Levels in Single Cell Populations" @default.
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• W2754396002 doi "https://doi.org/10.1007/978-1-4939-7240-1_10" @default.
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