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- W2754602914 abstract "Uridine phosphorylase is one of the critical enzymes in the pyrimidine salvage pathway. Cells regenerate uridine for nucleotide metabolism by incorporating uracil with ribose-1-phosphate with this enzyme. Recent studies indicate that Escherichia coli uridine phosphorylase is destabilized in the presence of ATP. However, the mechanism underlying the destabilization process and its influence on uridine phosphorylase function remain to be established. Here, we comprehensively investigated the effects of ATP on protein folding and function of Escherichia coli uridine phosphorylase. Our results demonstrate that ATP apparently decreases the stability of uridine phosphorylase in a concentration-dependent manner. Additionally, simply increasing the level of ATP led to a reduction of enzymatic activity to complete inhibition. Further studies showed that uridine phosphorylase accumulates as a partially unfolded state in the presence of ATP. Moreover, ATP specifically accelerated the unfolding rate of uridine phosphorylase with no observable effects on the refolding process. Our preliminary findings suggest that ATP can alter the protein folding and function of enzymes via apparent destabilization. This mechanism may be significant for proteins functioning under conditions of high levels of ATP, such as cancer cell environments." @default.
- W2754602914 created "2017-09-25" @default.
- W2754602914 creator A5005962077 @default.
- W2754602914 creator A5081667373 @default.
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- W2754602914 date "2017-11-01" @default.
- W2754602914 modified "2023-10-18" @default.
- W2754602914 title "ATP alters protein folding and function of Escherichia coli uridine phosphorylase" @default.
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- W2754602914 doi "https://doi.org/10.1016/j.abb.2017.09.007" @default.
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