Matches in SemOpenAlex for { <https://semopenalex.org/work/W2755131430> ?p ?o ?g. }
- W2755131430 abstract "ABSTRACT The filamentous fungus Trichoderma reesei is found predominantly in the tropics but also in more temperate regions, such as Europe, and is widely known as a producer of large amounts of plant cell wall-degrading enzymes. We sequenced the genome of the sexually competent isolate CBS999.97, which is phenotypically different from the female sterile strain QM6a but can cross sexually with QM6a. Transcriptome data for growth on cellulose showed that entire carbohydrate-active enzyme (CAZyme) families are consistently differentially regulated between these strains. We evaluated backcrossed strains of both mating types, which acquired female fertility from CBS999.97 but maintained a mostly QM6a genetic background, and we could thereby distinguish between the effects of strain background and female fertility or mating type. We found clear regulatory differences associated with female fertility and female sterility, including regulation of CAZyme and transporter genes. Analysis of carbon source utilization, transcriptomes, and secondary metabolites in these strains revealed that only a few changes in gene regulation are consistently correlated with different mating types. Different strain backgrounds (QM6a versus CBS999.97) resulted in the most significant alterations in the transcriptomes and in carbon source utilization, with decreased growth of CBS999.97 on several amino acids (for example proline or alanine), which further correlated with the downregulation of genes involved in the respective pathways. In combination, our findings support a role of fertility-associated processes in physiology and gene regulation and are of high relevance for the use of sexual crossing in combining the characteristics of two compatible strains or quantitative trait locus (QTL) analysis. IMPORTANCE Trichoderma reesei is a filamentous fungus with a high potential for secretion of plant cell wall-degrading enzymes. We sequenced the genome of the fully fertile field isolate CBS999.97 and analyzed its gene regulation characteristics in comparison with the commonly used laboratory wild-type strain QM6a, which is not female fertile. Additionally, we also evaluated fully fertile strains with genotypes very close to that of QM6a in order to distinguish between strain-specific and fertility-specific characteristics. We found that QM6a and CBS999.97 clearly differ in their growth patterns on different carbon sources, CAZyme gene regulation, and secondary metabolism. Importantly, we found altered regulation of 90 genes associated with female fertility, including CAZyme genes and transporter genes, but only minor mating type-dependent differences. Hence, when using sexual crossing in research and for strain improvement, it is important to consider female fertile and female sterile strains for comparison with QM6a and to achieve optimal performance." @default.
- W2755131430 created "2017-09-25" @default.
- W2755131430 creator A5000055209 @default.
- W2755131430 creator A5002514645 @default.
- W2755131430 creator A5002842402 @default.
- W2755131430 creator A5003978738 @default.
- W2755131430 creator A5016107215 @default.
- W2755131430 creator A5016906577 @default.
- W2755131430 creator A5042581605 @default.
- W2755131430 creator A5047488711 @default.
- W2755131430 creator A5053619689 @default.
- W2755131430 creator A5054440982 @default.
- W2755131430 creator A5064356229 @default.
- W2755131430 creator A5067761628 @default.
- W2755131430 creator A5068040575 @default.
- W2755131430 creator A5075067727 @default.
- W2755131430 creator A5083076589 @default.
- W2755131430 creator A5089883420 @default.
- W2755131430 date "2017-11-15" @default.
- W2755131430 modified "2023-10-17" @default.
- W2755131430 title "Omics Analyses of Trichoderma reesei CBS999.97 and QM6a Indicate the Relevance of Female Fertility to Carbohydrate-Active Enzyme and Transporter Levels" @default.
- W2755131430 cites W1635409144 @default.
- W2755131430 cites W1651207300 @default.
- W2755131430 cites W1826611623 @default.
- W2755131430 cites W186192844 @default.
- W2755131430 cites W1951320442 @default.
- W2755131430 cites W1972924519 @default.
- W2755131430 cites W1987332056 @default.
- W2755131430 cites W1987622511 @default.
- W2755131430 cites W1990073900 @default.
- W2755131430 cites W2009639464 @default.
- W2755131430 cites W2011218424 @default.
- W2755131430 cites W2011485738 @default.
- W2755131430 cites W2024296379 @default.
- W2755131430 cites W2033834490 @default.
- W2755131430 cites W2036875488 @default.
- W2755131430 cites W2045313171 @default.
- W2755131430 cites W2050604745 @default.
- W2755131430 cites W2055250487 @default.
- W2755131430 cites W2058877429 @default.
- W2755131430 cites W2061154517 @default.
- W2755131430 cites W2076448996 @default.
- W2755131430 cites W2080951000 @default.
- W2755131430 cites W2081190196 @default.
- W2755131430 cites W2093476095 @default.
- W2755131430 cites W2099259718 @default.
- W2755131430 cites W2109924970 @default.
- W2755131430 cites W2110596109 @default.
- W2755131430 cites W2113079985 @default.
- W2755131430 cites W2117910326 @default.
- W2755131430 cites W2121931648 @default.
- W2755131430 cites W2122220197 @default.
- W2755131430 cites W2124664561 @default.
- W2755131430 cites W2125377751 @default.
- W2755131430 cites W2129944256 @default.
- W2755131430 cites W2131224113 @default.
- W2755131430 cites W2137917513 @default.
- W2755131430 cites W2156688408 @default.
- W2755131430 cites W2162595238 @default.
- W2755131430 cites W2164686942 @default.
- W2755131430 cites W2165759219 @default.
- W2755131430 cites W2168506412 @default.
- W2755131430 cites W2262569837 @default.
- W2755131430 cites W2278317942 @default.
- W2755131430 cites W2419653649 @default.
- W2755131430 cites W2461914400 @default.
- W2755131430 cites W2481115317 @default.
- W2755131430 cites W2569773768 @default.
- W2755131430 cites W2613787240 @default.
- W2755131430 cites W2747527165 @default.
- W2755131430 cites W4251849943 @default.
- W2755131430 cites W74173866 @default.
- W2755131430 doi "https://doi.org/10.1128/aem.01578-17" @default.
- W2755131430 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5666144" @default.
- W2755131430 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28916559" @default.
- W2755131430 hasPublicationYear "2017" @default.
- W2755131430 type Work @default.
- W2755131430 sameAs 2755131430 @default.
- W2755131430 citedByCount "17" @default.
- W2755131430 countsByYear W27551314302018 @default.
- W2755131430 countsByYear W27551314302019 @default.
- W2755131430 countsByYear W27551314302020 @default.
- W2755131430 countsByYear W27551314302021 @default.
- W2755131430 countsByYear W27551314302022 @default.
- W2755131430 countsByYear W27551314302023 @default.
- W2755131430 crossrefType "journal-article" @default.
- W2755131430 hasAuthorship W2755131430A5000055209 @default.
- W2755131430 hasAuthorship W2755131430A5002514645 @default.
- W2755131430 hasAuthorship W2755131430A5002842402 @default.
- W2755131430 hasAuthorship W2755131430A5003978738 @default.
- W2755131430 hasAuthorship W2755131430A5016107215 @default.
- W2755131430 hasAuthorship W2755131430A5016906577 @default.
- W2755131430 hasAuthorship W2755131430A5042581605 @default.
- W2755131430 hasAuthorship W2755131430A5047488711 @default.
- W2755131430 hasAuthorship W2755131430A5053619689 @default.
- W2755131430 hasAuthorship W2755131430A5054440982 @default.
- W2755131430 hasAuthorship W2755131430A5064356229 @default.
- W2755131430 hasAuthorship W2755131430A5067761628 @default.
- W2755131430 hasAuthorship W2755131430A5068040575 @default.
- W2755131430 hasAuthorship W2755131430A5075067727 @default.