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- W2755580755 abstract "Nε-carboxymethyl-lysine (CML) is measured in food, but there is a controversy concerning the most convenient yet reliable method(s) for this task. This work compares three different ELISA assays and HPLC-ESI-ITMS/MS for the analysis of CML in several food items. The four methods showed the same decreasing order of CML concentration: beef, bacon > chicken > fish > dairy products > grain products > fruits/vegetables. HPLC-ESI-ITMS/MS results highly correlated with those obtained by ELISA performed with monoclonal CML-antibody (β = 0.98, p < 0.0001) whereas My Bio Source® kit results were not correlated with those provided by Lamider®. Small differences of CML concentrations in food items prepared by different culinary treatment were clearly distinguished by HPLC-ESI-ITMS/MS, but could not always be detected by ELISA. This work demonstrates a reasonable relationship between CM determined by ELISA and HPLC-ESI-ITMS/MS and therefore supports the implementation of ELISA in food CML/AGEs screening." @default.
- W2755580755 created "2017-09-25" @default.
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- W2755580755 date "2018-03-01" @default.
- W2755580755 modified "2023-10-18" @default.
- W2755580755 title "Comparative evaluation of three different ELISA assays and HPLC-ESI-ITMS/MS for the analysis of N ε -carboxymethyl lysine in food samples" @default.
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- W2755580755 doi "https://doi.org/10.1016/j.foodchem.2017.09.098" @default.
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