Matches in SemOpenAlex for { <https://semopenalex.org/work/W2755765087> ?p ?o ?g. }
- W2755765087 abstract "Light-gated ion channels and transporters have been applied to a broad array of excitable cells including neurons, cardiac myocytes, skeletal muscle cells and pancreatic β-cells in an organism to clarify their physiological and pathological roles. Nonetheless, among nonexcitable cells, only glial cells have been studied in vivo by this approach. Here, by optogenetic stimulation of a different nonexcitable cell type in the cochlea of the inner ear, we induce and control hearing loss. To our knowledge, deafness animal models using optogenetics have not yet been established. Analysis of transgenic mice expressing channelrhodopsin-2 (ChR2) induced by an oligodendrocyte-specific promoter identified this channel in nonglial cells-melanocytes-of an epithelial-like tissue in the cochlea. The membrane potential of these cells underlies a highly positive potential in a K+-rich extracellular solution, endolymph; this electrical property is essential for hearing. Illumination of the cochlea to activate ChR2 and depolarize the melanocytes significantly impaired hearing within a few minutes, accompanied by a reduction in the endolymphatic potential. After cessation of the illumination, the hearing thresholds and potential returned to baseline during several minutes. These responses were replicable multiple times. ChR2 was also expressed in cochlear glial cells surrounding the neuronal components, but slight neural activation caused by the optical stimulation was unlikely to be involved in the hearing impairment. The acute-onset, reversible and repeatable phenotype, which is inaccessible to conventional gene-targeting and pharmacological approaches, seems to at least partially resemble the symptom in a population of patients with sensorineural hearing loss. Taken together, this mouse line may not only broaden applications of optogenetics but also contribute to the progress of translational research on deafness." @default.
- W2755765087 created "2017-09-25" @default.
- W2755765087 creator A5001606341 @default.
- W2755765087 creator A5010251793 @default.
- W2755765087 creator A5019523801 @default.
- W2755765087 creator A5021824059 @default.
- W2755765087 creator A5026210334 @default.
- W2755765087 creator A5029978747 @default.
- W2755765087 creator A5030342167 @default.
- W2755765087 creator A5041303813 @default.
- W2755765087 creator A5041560935 @default.
- W2755765087 creator A5048212731 @default.
- W2755765087 creator A5054057203 @default.
- W2755765087 creator A5054653023 @default.
- W2755765087 creator A5057153260 @default.
- W2755765087 creator A5062455757 @default.
- W2755765087 creator A5062558610 @default.
- W2755765087 creator A5070435631 @default.
- W2755765087 creator A5070814339 @default.
- W2755765087 creator A5082886918 @default.
- W2755765087 creator A5083254719 @default.
- W2755765087 creator A5085080129 @default.
- W2755765087 creator A5089733324 @default.
- W2755765087 date "2017-09-21" @default.
- W2755765087 modified "2023-10-18" @default.
- W2755765087 title "Hearing Loss Controlled by Optogenetic Stimulation of Nonexcitable Nonglial Cells in the Cochlea of the Inner Ear" @default.
- W2755765087 cites W1139015881 @default.
- W2755765087 cites W1520600324 @default.
- W2755765087 cites W1893121599 @default.
- W2755765087 cites W1916040231 @default.
- W2755765087 cites W1938365946 @default.
- W2755765087 cites W1963675274 @default.
- W2755765087 cites W1963976166 @default.
- W2755765087 cites W1968350683 @default.
- W2755765087 cites W1970498119 @default.
- W2755765087 cites W1970867422 @default.
- W2755765087 cites W1979085769 @default.
- W2755765087 cites W1983818494 @default.
- W2755765087 cites W2000879278 @default.
- W2755765087 cites W2001717951 @default.
- W2755765087 cites W2005872411 @default.
- W2755765087 cites W2006655162 @default.
- W2755765087 cites W2010368222 @default.
- W2755765087 cites W2010577228 @default.
- W2755765087 cites W2015106084 @default.
- W2755765087 cites W2018586643 @default.
- W2755765087 cites W2019361699 @default.
- W2755765087 cites W2023230481 @default.
- W2755765087 cites W2024751118 @default.
- W2755765087 cites W2028456320 @default.
- W2755765087 cites W2035928348 @default.
- W2755765087 cites W2042143125 @default.
- W2755765087 cites W2045659867 @default.
- W2755765087 cites W2049069390 @default.
- W2755765087 cites W2056173031 @default.
- W2755765087 cites W2057723728 @default.
- W2755765087 cites W2058569281 @default.
- W2755765087 cites W2061559989 @default.
- W2755765087 cites W2061677892 @default.
- W2755765087 cites W2062977802 @default.
- W2755765087 cites W2073043548 @default.
- W2755765087 cites W2073925985 @default.
- W2755765087 cites W2075001495 @default.
- W2755765087 cites W2080028500 @default.
- W2755765087 cites W2081164052 @default.
- W2755765087 cites W2082823927 @default.
- W2755765087 cites W2093492175 @default.
- W2755765087 cites W2097413823 @default.
- W2755765087 cites W2098801459 @default.
- W2755765087 cites W2107702642 @default.
- W2755765087 cites W2110201885 @default.
- W2755765087 cites W2121276197 @default.
- W2755765087 cites W2123050929 @default.
- W2755765087 cites W2142077813 @default.
- W2755765087 cites W2142087766 @default.
- W2755765087 cites W2146247758 @default.
- W2755765087 cites W2146882075 @default.
- W2755765087 cites W2146961403 @default.
- W2755765087 cites W2164049077 @default.
- W2755765087 cites W2166763307 @default.
- W2755765087 cites W2168819411 @default.
- W2755765087 cites W223107217 @default.
- W2755765087 cites W2407725967 @default.
- W2755765087 cites W2412215475 @default.
- W2755765087 cites W2415617471 @default.
- W2755765087 cites W2474442576 @default.
- W2755765087 cites W2498257067 @default.
- W2755765087 cites W2508930197 @default.
- W2755765087 cites W2642373867 @default.
- W2755765087 cites W4245492581 @default.
- W2755765087 doi "https://doi.org/10.3389/fnmol.2017.00300" @default.
- W2755765087 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5616010" @default.
- W2755765087 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29018325" @default.
- W2755765087 hasPublicationYear "2017" @default.
- W2755765087 type Work @default.
- W2755765087 sameAs 2755765087 @default.
- W2755765087 citedByCount "2" @default.
- W2755765087 countsByYear W27557650872020 @default.
- W2755765087 countsByYear W27557650872022 @default.
- W2755765087 crossrefType "journal-article" @default.