Matches in SemOpenAlex for { <https://semopenalex.org/work/W2756022268> ?p ?o ?g. }
Showing items 1 to 56 of
56
with 100 items per page.
- W2756022268 endingPage "3334" @default.
- W2756022268 startingPage "3327" @default.
- W2756022268 abstract "为探究抗生素和重金属对抗性转移及接合子抗性基因丰度的影响，借助绿色荧光蛋白标记质粒，采用滤膜接合配对方法研究了胁迫条件对细菌接合频率的影响，定量检测了转移后的AmpC β-内酰胺酶类抗性基因丰度的变化.结果表明，头孢噻肟钠及重金属共胁迫促进了AmpC β-内酰胺酶类抗性基因从复杂群落到Escherichia coli BL21的转移.3 μg·L-1头孢噻肟钠胁迫下，细菌接合频率为空白组的2.06倍，其中，接合子耐药基因CIT、FOX和EBC的相对丰度分别升高了7.47、4.30和1.60倍.同时，以3 μg·L-1头孢噻肟钠胁迫组为对照，研究了重金属汞、铜和锌共胁迫对抗性转移的影响，考虑到头孢噻肟钠的水解特性和接合子克隆性扩增，接合时间定为3 h.结果发现，汞、铜、锌与头孢噻肟钠共胁迫组的细菌接合频率分别比对照组增长了2.57、1.60和1.74倍.汞共胁迫对接合子中7种AmpC β-内酰胺酶类抗性基因丰度无显著影响；铜共胁迫效应因抗生素抗性基因（ARGs）种类不同而不同，其中，对抗性基因EBC相对丰度的影响最大；锌共胁迫效应受锌离子浓度和ARGs种类影响，100 mg·L-1锌共胁迫使得EBC和CIT丰度比对照组分别增加3.56和2.76倍.可见，抗生素与重金属共胁迫条件可促进环境中AmpC β-内酰胺酶类抗性基因的转移，值得关注." @default.
- W2756022268 created "2017-09-25" @default.
- W2756022268 creator A5001167034 @default.
- W2756022268 creator A5011812706 @default.
- W2756022268 creator A5023250795 @default.
- W2756022268 creator A5028965081 @default.
- W2756022268 creator A5032393271 @default.
- W2756022268 creator A5045369158 @default.
- W2756022268 creator A5048226043 @default.
- W2756022268 creator A5060335470 @default.
- W2756022268 creator A5065380119 @default.
- W2756022268 creator A5071218319 @default.
- W2756022268 creator A5072580346 @default.
- W2756022268 creator A5074022512 @default.
- W2756022268 creator A5076118607 @default.
- W2756022268 creator A5076876979 @default.
- W2756022268 creator A5087055672 @default.
- W2756022268 creator A5091596489 @default.
- W2756022268 date "2017-09-06" @default.
- W2756022268 modified "2023-09-28" @default.
- W2756022268 title "头孢噻肟钠与重金属对AmpC β-内酰胺酶类抗性基因转移的影响" @default.
- W2756022268 doi "https://doi.org/10.13671/j.hjkxxb.2017.0048" @default.
- W2756022268 hasPublicationYear "2017" @default.
- W2756022268 type Work @default.
- W2756022268 sameAs 2756022268 @default.
- W2756022268 citedByCount "0" @default.
- W2756022268 crossrefType "journal-article" @default.
- W2756022268 hasAuthorship W2756022268A5001167034 @default.
- W2756022268 hasAuthorship W2756022268A5011812706 @default.
- W2756022268 hasAuthorship W2756022268A5023250795 @default.
- W2756022268 hasAuthorship W2756022268A5028965081 @default.
- W2756022268 hasAuthorship W2756022268A5032393271 @default.
- W2756022268 hasAuthorship W2756022268A5045369158 @default.
- W2756022268 hasAuthorship W2756022268A5048226043 @default.
- W2756022268 hasAuthorship W2756022268A5060335470 @default.
- W2756022268 hasAuthorship W2756022268A5065380119 @default.
- W2756022268 hasAuthorship W2756022268A5071218319 @default.
- W2756022268 hasAuthorship W2756022268A5072580346 @default.
- W2756022268 hasAuthorship W2756022268A5074022512 @default.
- W2756022268 hasAuthorship W2756022268A5076118607 @default.
- W2756022268 hasAuthorship W2756022268A5076876979 @default.
- W2756022268 hasAuthorship W2756022268A5087055672 @default.
- W2756022268 hasAuthorship W2756022268A5091596489 @default.
- W2756022268 hasConcept C41008148 @default.
- W2756022268 hasConceptScore W2756022268C41008148 @default.
- W2756022268 hasIssue "9" @default.
- W2756022268 hasLocation W27560222681 @default.
- W2756022268 hasOpenAccess W2756022268 @default.
- W2756022268 hasPrimaryLocation W27560222681 @default.
- W2756022268 hasVolume "37" @default.
- W2756022268 isParatext "false" @default.
- W2756022268 isRetracted "false" @default.
- W2756022268 magId "2756022268" @default.
- W2756022268 workType "article" @default.