Matches in SemOpenAlex for { <https://semopenalex.org/work/W2756154183> ?p ?o ?g. }
- W2756154183 abstract "Campylobacter jejuni is the leading cause of campylobacteriosis in the developed world. Although most cases are caused by consumption of contaminated meat, a significant proportion is linked to ingestion of contaminated water. The differences between C. jejuni strains originating from food products and those isolated from water are poorly understood. Working under the hypothesis that water-borne C. jejuni strains are better equipped at surviving the nutrient-poor aquatic environment than food-borne strains, the present study aims to characterize these differences using outbreak strains 81116 and 81-176. Strain 81116 caused a campylobacteriosis outbreak linked to consumption of water, while strain 81-176 was linked to consumption of raw milk. CFU counts and viability assays showed that 81116 survives better than 81-176 at 4°C in a defined freshwater medium (Fraquil). Moreover, 81116 was significantly more resistant to oxidative stress and bile salt than strain 81-176 in Fraquil. To better understand the genetic response of 81116 to water, a transcriptomic profiling study was undertaken using microarrays. Compared to rich broth, strain 81116 represses genes involved in amino acid uptake and metabolism, as well as genes involved in costly biosynthetic processes such as replication, translation, flagellum synthesis and virulence in response to Fraquil. In accordance with the observed increase in stress resistance in Fraquil, 81116 induces genes involved in resistance to oxidative stress and bile salt. Interestingly, genes responsible for cell wall synthesis were also induced upon Fraquil exposure. Finally, twelve unique genes were expressed in Fraquil; however, analysis of their distribution in animal and water isolates showed that they are not uniquely and ubiquitously present in water isolates, and thus, unlikely to play a major role in adaptation to water. Our results show that some C. jejuni strains are more resilient than others, thereby challenging current water management practices. The response of 81116 to Fraquil serves as a starting point to understand the adaptation of C. jejuni to water and its subsequent transmission." @default.
- W2756154183 created "2017-09-25" @default.
- W2756154183 creator A5009912584 @default.
- W2756154183 creator A5015742887 @default.
- W2756154183 creator A5049788459 @default.
- W2756154183 creator A5061262449 @default.
- W2756154183 creator A5068286424 @default.
- W2756154183 creator A5078973829 @default.
- W2756154183 creator A5080289774 @default.
- W2756154183 creator A5087033539 @default.
- W2756154183 date "2017-09-20" @default.
- W2756154183 modified "2023-10-03" @default.
- W2756154183 title "Phenotypic and Transcriptomic Responses of Campylobacter jejuni Suspended in an Artificial Freshwater Medium" @default.
- W2756154183 cites W1485869082 @default.
- W2756154183 cites W1487634705 @default.
- W2756154183 cites W1519779150 @default.
- W2756154183 cites W1554968268 @default.
- W2756154183 cites W1574727116 @default.
- W2756154183 cites W1754108693 @default.
- W2756154183 cites W1768217943 @default.
- W2756154183 cites W1778633979 @default.
- W2756154183 cites W1780745691 @default.
- W2756154183 cites W1921670651 @default.
- W2756154183 cites W1964398610 @default.
- W2756154183 cites W1967192362 @default.
- W2756154183 cites W1967536176 @default.
- W2756154183 cites W1969043085 @default.
- W2756154183 cites W1976689351 @default.
- W2756154183 cites W1978480500 @default.
- W2756154183 cites W1984409531 @default.
- W2756154183 cites W1989446695 @default.
- W2756154183 cites W1992042174 @default.
- W2756154183 cites W1994810822 @default.
- W2756154183 cites W1997782608 @default.
- W2756154183 cites W1999890791 @default.
- W2756154183 cites W2002807448 @default.
- W2756154183 cites W2004494938 @default.
- W2756154183 cites W2004787320 @default.
- W2756154183 cites W2014557276 @default.
- W2756154183 cites W2020077180 @default.
- W2756154183 cites W2020332632 @default.
- W2756154183 cites W2020347152 @default.
- W2756154183 cites W2021519267 @default.
- W2756154183 cites W2023177772 @default.
- W2756154183 cites W2027825927 @default.
- W2756154183 cites W2031713442 @default.
- W2756154183 cites W2033756681 @default.
- W2756154183 cites W2035215035 @default.
- W2756154183 cites W2038629035 @default.
- W2756154183 cites W2042458706 @default.
- W2756154183 cites W2043805760 @default.
- W2756154183 cites W2045423564 @default.
- W2756154183 cites W2049796015 @default.
- W2756154183 cites W2051990646 @default.
- W2756154183 cites W2063128850 @default.
- W2756154183 cites W2064059801 @default.
- W2756154183 cites W2064930241 @default.
- W2756154183 cites W2070791088 @default.
- W2756154183 cites W2073575565 @default.
- W2756154183 cites W2073988387 @default.
- W2756154183 cites W2074466419 @default.
- W2756154183 cites W2074792363 @default.
- W2756154183 cites W2079081483 @default.
- W2756154183 cites W2079617293 @default.
- W2756154183 cites W2082819719 @default.
- W2756154183 cites W2086107275 @default.
- W2756154183 cites W2087664087 @default.
- W2756154183 cites W2096078976 @default.
- W2756154183 cites W2101220987 @default.
- W2756154183 cites W2107277218 @default.
- W2756154183 cites W2108336065 @default.
- W2756154183 cites W2108368634 @default.
- W2756154183 cites W2110768122 @default.
- W2756154183 cites W2115525385 @default.
- W2756154183 cites W2117875503 @default.
- W2756154183 cites W2117927148 @default.
- W2756154183 cites W2119121117 @default.
- W2756154183 cites W2119537314 @default.
- W2756154183 cites W2120904752 @default.
- W2756154183 cites W2123244912 @default.
- W2756154183 cites W2123924466 @default.
- W2756154183 cites W2125591244 @default.
- W2756154183 cites W2127179936 @default.
- W2756154183 cites W2127242142 @default.
- W2756154183 cites W2128981277 @default.
- W2756154183 cites W2128984003 @default.
- W2756154183 cites W2134141380 @default.
- W2756154183 cites W2134162213 @default.
- W2756154183 cites W2134809564 @default.
- W2756154183 cites W2135973921 @default.
- W2756154183 cites W2138844936 @default.
- W2756154183 cites W2139310472 @default.
- W2756154183 cites W2143364665 @default.
- W2756154183 cites W2144200574 @default.
- W2756154183 cites W2147075264 @default.
- W2756154183 cites W2147746168 @default.
- W2756154183 cites W2150180261 @default.
- W2756154183 cites W2154956953 @default.
- W2756154183 cites W2162140510 @default.
- W2756154183 cites W2162392176 @default.