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• W2756247668 abstract "Background: Two cembranoid diterpenes, [(1 S , 2 E , 4 R , 6 R , 7 E , 11 E )-2, 7, 11-cembratriene-4, 6-diol] ( 1 ) and its epimer [(1 S , 2 E , 4 S , 6 R ,7 E ,11 E )-2, 7, 11-cembratriene-4, 6-diol] ( 2 ) were isolated from surface dichloromethane washings and chloroform extract of Nicotiana sylvestris leaves. Methods: The compounds were purified using silica gel column- thin layer- and flash column chromatography methods. The structures of the isolated compounds were elucidated using spectroscopic analysis and their 1 H and 13 C NMR spectroscopic data were compared with those of authentic samples reported in the literature. The cytotoxic activity of 1 and 2 against three human cancer cell lines, including LS180 (human colon adenocarcinoma), MCF-7 (human breast adenocarcinoma) and MOLT-4 (human lymphoblastic leukemia) were evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric bioassay. Results: The IC 50 values of compounds 1 and 2 were calculated to be between (28.4±3.7 up to 44.0±6.4 µM) (mean±S.E.M.) for the above mentioned cell lines." @default.
• W2756247668 created "2017-09-25" @default.
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• W2756247668 date "2017-09-21" @default.
• W2756247668 modified "2023-10-03" @default.
• W2756247668 title "Cytotoxic Activity of Two Cembranoid Diterpenes from Nicotiana Sylvestris Against Three Human Cancer Cell Lines" @default.
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• W2756247668 doi "https://doi.org/10.2174/1874847301705010001" @default.
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