FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2756260059> ?p ?o ?g. }

• W2756260059 abstract "ABSTRACT The pst gene cluster encodes the phosphate-specific transport (Pst) system. Inactivation of the Pst system constitutively activates the two-component regulatory system PhoBR and attenuates the virulence of pathogenic bacteria. In uropathogenic Escherichia coli strain CFT073, attenuation by inactivation of pst is predominantly attributed to the decreased expression of type 1 fimbriae. However, the molecular mechanisms connecting the Pst system and type 1 fimbriae are unknown. To address this, a transposon library was constructed in the pst mutant, and clones were tested for a regain in type 1 fimbrial production. Among them, the diguanylate cyclase encoded by yaiC ( adrA in Salmonella ) was identified to connect the Pst system and type 1 fimbrial expression. In the pst mutant, the decreased expression of type 1 fimbriae is connected by the induction of yaiC . This is predominantly due to altered expression of the FimBE-like recombinase genes ipuA and ipbA , affecting at the same time the inversion of the fim promoter switch ( fimS ). In the pst mutant, inactivation of yaiC restored fim -dependent adhesion to bladder cells and virulence. Interestingly, the expression of yaiC was activated by PhoB, since transcription of yaiC was linked to the PhoB-dependent phoA-psiF operon. As YaiC is involved in cyclic di-GMP (c-di-GMP) biosynthesis, an increased accumulation of c-di-GMP was observed in the pst mutant. Hence, the results suggest that one mechanism by which deletion of the Pst system reduces the expression of type 1 fimbriae is through PhoBR-mediated activation of yaiC , which in turn increases the accumulation of c-di-GMP, represses the fim operon, and, consequently, attenuates virulence in the mouse urinary tract infection model. IMPORTANCE Urinary tract infections (UTIs) are common bacterial infections in humans. They are mainly caused by uropathogenic Escherichia coli (UPEC). We previously showed that interference with phosphate homeostasis decreases the expression of type 1 fimbriae and attenuates UPEC virulence. Herein, we identified that alteration of the phosphate metabolism increases production of the signaling molecule c-di-GMP, which in turn decreases the expression of type 1 fimbriae. We also determine the regulatory cascade leading to the accumulation of c-di-GMP and identify the Pho regulon as new players in c-di-GMP-mediated cell signaling. By understanding the molecular mechanisms leading to the expression of virulence factors, we will be in a better position to develop new therapeutics." @default.
• W2756260059 created "2017-09-25" @default.
• W2756260059 creator A5006528926 @default.
• W2756260059 creator A5009832075 @default.
• W2756260059 creator A5030542493 @default.
• W2756260059 creator A5059182343 @default.
• W2756260059 creator A5065155909 @default.
• W2756260059 date "2017-12-15" @default.
• W2756260059 modified "2023-10-16" @default.
• W2756260059 title "Altered Regulation of the Diguanylate Cyclase YaiC Reduces Production of Type 1 Fimbriae in a Pst Mutant of Uropathogenic Escherichia coli CFT073" @default.
• W2756260059 cites W1204073589 @default.
• W2756260059 cites W1499426843 @default.
• W2756260059 cites W1500734314 @default.
• W2756260059 cites W1579696096 @default.
• W2756260059 cites W1786268878 @default.
• W2756260059 cites W1824614244 @default.
• W2756260059 cites W1833844910 @default.
• W2756260059 cites W1906639127 @default.
• W2756260059 cites W1921921966 @default.
• W2756260059 cites W1963867445 @default.
• W2756260059 cites W1968750111 @default.
• W2756260059 cites W1971023242 @default.
• W2756260059 cites W1975415672 @default.
• W2756260059 cites W1976381180 @default.
• W2756260059 cites W1984136937 @default.
• W2756260059 cites W1990722231 @default.
• W2756260059 cites W2002388494 @default.
• W2756260059 cites W2002648188 @default.
• W2756260059 cites W2003014014 @default.
• W2756260059 cites W2014361215 @default.
• W2756260059 cites W2015081806 @default.
• W2756260059 cites W2020455774 @default.
• W2756260059 cites W2023956150 @default.
• W2756260059 cites W2035856903 @default.
• W2756260059 cites W2046721427 @default.
• W2756260059 cites W2047353791 @default.
• W2756260059 cites W2053138162 @default.
• W2756260059 cites W2053812068 @default.
• W2756260059 cites W2061036236 @default.
• W2756260059 cites W2062879226 @default.
• W2756260059 cites W2072862102 @default.
• W2756260059 cites W2082668323 @default.
• W2756260059 cites W2083275944 @default.
• W2756260059 cites W2093021888 @default.
• W2756260059 cites W2096786316 @default.
• W2756260059 cites W2106702513 @default.
• W2756260059 cites W2107277218 @default.
• W2756260059 cites W2109335319 @default.
• W2756260059 cites W2116137883 @default.
• W2756260059 cites W2116985181 @default.
• W2756260059 cites W2120681623 @default.
• W2756260059 cites W2123803344 @default.
• W2756260059 cites W2131036374 @default.
• W2756260059 cites W2131051901 @default.
• W2756260059 cites W2132665880 @default.
• W2756260059 cites W2140440247 @default.
• W2756260059 cites W2141396945 @default.
• W2756260059 cites W2141421676 @default.
• W2756260059 cites W2142721630 @default.
• W2756260059 cites W2143106471 @default.
• W2756260059 cites W2144498842 @default.
• W2756260059 cites W2147185759 @default.
• W2756260059 cites W2149601262 @default.
• W2756260059 cites W2150721936 @default.
• W2756260059 cites W2153139855 @default.
• W2756260059 cites W2154075906 @default.
• W2756260059 cites W2154203929 @default.
• W2756260059 cites W2157620368 @default.
• W2756260059 cites W2158345344 @default.
• W2756260059 cites W2160529598 @default.
• W2756260059 cites W2161831451 @default.
• W2756260059 cites W2165490685 @default.
• W2756260059 cites W2166970960 @default.
• W2756260059 cites W2166997758 @default.
• W2756260059 cites W2167356927 @default.
• W2756260059 cites W2167600249 @default.
• W2756260059 cites W2169220413 @default.
• W2756260059 cites W2171132114 @default.
• W2756260059 cites W2192080449 @default.
• W2756260059 cites W2296481779 @default.
• W2756260059 cites W2339829243 @default.
• W2756260059 cites W2439883537 @default.
• W2756260059 cites W2586995851 @default.
• W2756260059 cites W783470056 @default.
• W2756260059 doi "https://doi.org/10.1128/jb.00168-17" @default.
• W2756260059 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5686606" @default.
• W2756260059 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28924030" @default.
• W2756260059 hasPublicationYear "2017" @default.
• W2756260059 type Work @default.
• W2756260059 sameAs 2756260059 @default.
• W2756260059 citedByCount "11" @default.
• W2756260059 countsByYear W27562600592019 @default.
• W2756260059 countsByYear W27562600592020 @default.
• W2756260059 countsByYear W27562600592021 @default.
• W2756260059 countsByYear W27562600592022 @default.
• W2756260059 countsByYear W27562600592023 @default.
• W2756260059 crossrefType "journal-article" @default.
• W2756260059 hasAuthorship W2756260059A5006528926 @default.
• W2756260059 hasAuthorship W2756260059A5009832075 @default.
• W2756260059 hasAuthorship W2756260059A5030542493 @default.

• next